iTRAQ analysis of Singapore grouper iridovirus infection in a grouper embryonic cell line
iTRAQ analysis of Singapore grouper iridovirus infection in a grouper embryonic cell line
复制标题
DOI：
10.1099/vir.0.2008/003681-0
复制
发表时间：
2008-11-01
影响因子：
3.8
通讯作者：
Hew, Choy-Leong
中科院分区：
文献类型：
--
作者：
Chen, Li Ming;Tran, Bich Ngoc;Hew, Choy-Leong
我们报告，在这里，第一个蛋白质组学研究石斑鱼胚胎细胞系（GEC）感染新加坡石斑鱼虹彩病毒（SGIV）。采用iTRAQ标记，然后采用液相色谱/串联质谱法（LC-MS/MS）研究和定量了有和无病毒感染的GEC差异蛋白质组。共鉴定出49种病毒蛋白，其中11种为首次鉴定。此外，743个宿主蛋白质被揭示，并分为218个独特的蛋白质组。病毒感染后，14种宿主蛋白上调，5种宿主蛋白下调。GEC中SGIV感染的iTRAQ分析提供了在蛋白质水平上对病毒和宿主基因产物的洞察。这将有助于进一步研究和理解病毒与宿主的相互作用，病毒感染的分子机制和发病机制。
We report, here, the first proteomics study of a grouper embryonic cell line (GEC) infected by Singapore grouper iridovirus (SGIV). The differential proteomes of GEC with and without viral infection were studied and quantified with iTRAQ labelling followed by liquid chromatography/tandem mass spectrometry (LC-MS/MS). Forty-nine viral proteins were recognized, of which 11 were identified for the first time. Moreover, 743 host proteins were revealed and classified into 218 unique protein groups. Fourteen host proteins were upregulated and five host proteins were downregulated upon viral infection. The iTRAQ analysis of SGIV infection in GEC provides an insight to viral and host gene products at the protein level. This should facilitate further study and the understanding of virus-host interactions, molecular mechanisms of viral infection and pathogenesis.