U2AF HOMOLOG REQUIRED FOR SPLICING IN-VIVO
U2AF HOMOLOG REQUIRED FOR SPLICING IN-VIVO
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DOI：
10.1126/science.8211184
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发表时间：
1993-10-22
期刊：
影响因子：
56.9
通讯作者：
WENTZHUNTER, K
中科院分区：
文献类型：
--
作者：
POTASHKIN, J;NAIK, K;WENTZHUNTER, K
已经鉴定了几种在非允许温度下前mRNA加工缺陷的裂变酵母温度敏感突变体（prp突变体）。在这里，prp2+基因已被克隆，其能力，以弥补prp2突变体的温度敏感性生长缺陷。该基因还纠正prp2突变体的前mRNA剪接缺陷，并编码59千道尔顿多肽（PRP2）。一个分子表征表明，PRP2是一个以前未知的酵母剪接因子与哺乳动物剪接因子U2AF65的广泛相似性。因此，这项研究提供了证据表明，U2AF同源物参与体内RNA加工。
Several fission yeast temperature-sensitive mutants defective in pre-mRNA processing (prp- mutants) at the nonpermissive temperature have been identified. Here, the prp2+ gene has been cloned by its ability to complement the temperature-sensitive growth defect of a prp2- mutant. The gene also corrects the pre-mRNA splicing defect of prp2- mutants and encodes a 59-kilodalton polypeptide (PRP2). A molecular characterization indicates that PRP2 is a previously uncharacterized yeast splicing factor with extensive similarity to the mammalian splicing factor U2AF65. Thus, this study provides evidence that a U2AF homolog participates in RNA processing in vivo.