MOBILE PHASE SELECTION FOR THE HIGH-PERFORMANCE ION-EXCHANGE CHROMATOGRAPHY OF PROTEINS
MOBILE PHASE SELECTION FOR THE HIGH-PERFORMANCE ION-EXCHANGE CHROMATOGRAPHY OF PROTEINS
复制标题
DOI：
10.1016/0003-2697(83)90251-8
复制
发表时间：
1983-01-01
影响因子：
2.9
通讯作者：
REGNIER, FE
中科院分区：
文献类型：
--
作者：
KOPACIEWICZ, W;REGNIER, FE
适当的移动的相选择显着提高了蛋白质的高性能离子交换分馏。洗脱液的pH和盐含量影响强和弱离子交换柱上的色谱行为。在强和弱离子交换支持物上检查了一些蛋白质的保留和分辨率，这些移动的相变量。强离子交换柱是上级的蛋白质分离在这项研究中进行。弱离子交换柱和强离子交换柱的选择性是pH依赖性的;然而，强离子交换柱在更宽的pH范围内是可操作的。盐的影响的检查表明，它们的置换活性可以分为3类：弱，中间和强的蛋白质混合物。中间体盐提供了最好的分离度沿着良好的回收率。强置换盐可用于洗脱强烈保留的蛋白质。相对于蛋白质保留的移动的相的选择进行了讨论。
Proper mobile phase selection significantly improved high-performance ion-exchange fractionations of proteins. The pH and salt content of the eluant affected chromatographic behavior on both strong and weak ion-exchange columns. The retention and resolution of a number of proteins was examined on strong and weak ion-exchange supports with regard to these mobile phase variables. The strong ion-exchange columns were superior for the protein separations performed in this study. The selectivity of both weak and strong ion-exchange columns was pH dependent; however, strong ion-exchange columns were operable over a broader range of pH. Examination of the effect of salts demonstrated that their displacing activity could be divided into 3 categories: weak, intermediate and strong with regard to the protein mixture used. Intermediate salts provided best resolution along with good recoveries. Strong displacing salts were useful for eluting strongly retained proteins. The selection of a mobile phase with respect to protein retention is discussed.