Likelihood that an Unsubtypeable Influenza A Virus Result Obtained with the Luminex xTAG Respiratory Virus Panel Is Indicative of Infection with Novel A/H1N1 (Swine-Like) Influenza Virus
Likelihood that an Unsubtypeable Influenza A Virus Result Obtained with the Luminex xTAG Respiratory Virus Panel Is Indicative of Infection with Novel A/H1N1 (Swine-Like) Influenza Virus
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DOI：
10.1128/jcm.01027-09
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发表时间：
2009-07-01
影响因子：
9.4
通讯作者：
St. George, Kirsten
中科院分区：
文献类型：
--
作者：
Ginocchio, Christine C.;St. George, Kirsten
在季节性H1N1和H3 N2流感病毒同时流行的背景下，随着新型A/H1N1（猪样）流感病毒的突然出现，诊断实验室在敏感和准确检测方面面临困难。这种情况已进一步复杂化的可变的耐药模式在三个循环亚型，使具体的诊断重要的治疗和预防，以及流行病学的跟踪purposes.Many快速酶免疫分析方法已被发现是不可靠的检测新菌株，和延迟在获得的结果与文化，使这些方法在许多临床和公共卫生情况下的最佳。分子扩增检测已经是，并将继续是，在当前情况下，流感病毒的检测和分型的方法的选择。许多实验室缺乏内部开发分子检测试剂的能力，目前Luminex xTAG呼吸道病毒检测试剂盒（RVP）检测试剂（Luminex Molecular Diagnostics，多伦多，加拿大）是唯一一种经食品药品监督管理局批准的用于区分季节性甲型流感病毒亚型的检测试剂。RVP检测试剂通过扩增基质基因上的靶区域来检测甲型流感病毒，并通过使用对这些血凝素基因具有特异性的引物和探针来区分季节性H1和H3病毒亚型（2，3）。此外，该检测试剂盒可在单一反应中检测腺病毒、偏肺病毒、流感B病毒、副流感病毒1、2和3、鼻病毒和呼吸道合胞病毒。该测定提供的高度多路复用使得能够有效地使用样品，并使相对于输出的劳动最小化（1）。
With the sudden emergence of novel A/H1N1 (swine-like) inffuenza virus against a background of concurrently circulating seasonal H1N1 and H3N2 inffuenza viruses, diagnostic laboratories have been placed in a difficult situation regarding sensitive and accurate detection. This situation has been further complicated by the variable resistance patterns across the three circulating subtypes, making specific diagnosis important for treatment and prophylaxis, as well as for epidemiological tracking purposes.Many rapid enzyme immunoassay methods have been found to be unreliable for the detection of the new strain, and the delay in results obtainable with culture makes these methods suboptimal in many clinical and public health situations. Molecular amplification assays have been, and continue to be, the method of choice for detection and subtyping of inffuenza viruses in the current situation. Many laboratories lack the capability of developing molecular assays in-house, and currently the Luminex xTAG respiratory virus panel (RVP) assay (Luminex Molecular Diagnostics, Toronto, Canada) is the only Food and Drug Administration-approved assay that distinguishes the seasonal inffuenza A virus subtypes. The RVP assay detects inffuenza A virus by amplification of a target region on the matrix gene, and it distinguishes seasonal H1 and H3 virus subtypes by the use of primers and probes specific for those hemagglutinin genes (2, 3). In addition, the assay detects adenovirus, metapneumovirus, inffuenza B virus, parainffuenza viruses 1, 2, and 3, rhinovirus, and respiratory syncytial virus in a single reaction. The high degree of multiplexing afforded by the assay enables efficient use of sample and minimizes labor relative to the output (1).