The Arabidopsis thaliana MERISTEM LAYER 1 promoter specifies epidermal expression in meristems and young primordia
The Arabidopsis thaliana MERISTEM LAYER 1 promoter specifies epidermal expression in meristems and young primordia
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DOI：
10.1046/j.1365-313x.1999.00594.x
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发表时间：
1999-10-01
期刊：
影响因子：
7.2
通讯作者：
Yanofsky, MF
中科院分区：
文献类型：
--
作者：
Sessions, A;Weigel, D;Yanofsky, MF
拟南芥分生组织层1（ATML1）基因在发育中的胚和茎分生组织的表皮中表达。为了鉴定表皮特异性表达所需的调控序列，将三个重叠的ATML1基因组序列与GUS报告基因的融合物引入拟南芥植物中。所有的融合基因赋予表皮特异性表达的GUS mRNA和蛋白质活性，但不同的时间和相对水平的表达，这表明部分冗余的ATML1调控元件。这项研究定义了L1特异性调控序列，足以指导外源基因表达的层特异性的方式。
The Arabidopsis thaliana MERISTEM LAYER 1 (ATML1) gene is expressed in the epidermis of developing embryos and shoot meristems. To identify regulatory sequences necessary for epidermis-specific expression, three fusions of overlapping ATML1 genomic sequences to the GUS reporter gene were introduced into Arabidopsis plants. All fusion genes conferred epidermis-specific expression of both GUS mRNA and protein activity but varied in both the timing and relative levels of expression, suggesting partial redundancy of ATML1 regulatory elements. This study defines L1-specific regulatory sequences that are sufficient to direct foreign gene expression in a layer-specific manner.