Bioprocess optimization for biomass production of probiotics yeast Saccharomyces boulardii in semi-industrial scale.
Bioprocess optimization for biomass production of probiotics yeast Saccharomyces boulardii in semi-industrial scale.
复制标题
半工业规模益生菌布拉酵母菌生物质生产的生物工艺优化。
DOI：
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
H. El
中科院分区：
文献类型：
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作者：
T. Chin;N. Othman;R. Malek;Nagib A. Elmarzugi;Leng OngMei;Solleh Ramli;N. Musa;R. Aziz;H. El
布拉氏酵母菌是一种非致病性益生菌酵母，具有许多健康益处。在目前的工作中，进行了研究以改善细胞生长动力学，以半工业规模生产这种生物治疗酵母的细胞团。首先，筛选了五种不同的培养基，以选择最适合生物质生产的配方。选择产生最高细胞质量（2.57 g L -1 ）和最低乙醇含量的培养基，并通过改变每种组分的浓度进一步优化。优化后的培养基组成为(g·L)：葡萄糖，20；玉米浆，15；硝酸钠3，1.0； KH2PO4，6.0； MgSO4.7H20，3.0； CuSO4.5H20，0.002； FeSO4.7H20，0.001；然后使用 ZnSO4.7H2O, 0.01 在中试规模的 16 L 生物反应器中，在受控和非受控 pH 条件下进行分批培养，用于酵母生物质生产。在pH控制培养中获得了8.2 g L -1 的最高生物量。这几乎是摇瓶中获得的生物量的两倍。总之，我们已经开发出半工业规模的生物治疗酵母布拉氏酵母的高细胞量生产工艺。
Saccharomyces boulardii is non-pathogenic probiotic yeast with many health benefits. In the present wo rk, studies were carried out to improve cell growth kinetics to produce cell mass of this biotherapeutic yeast in semi-industrial scale. At first, five different media were screened to select the most appropriate formula for biomass production. The medium which yielded highest cell mass of 2.57 g L -1 and lowest ethanol was selected and further optimi zed by changing the concentrations of each component. The optimized medium composed of (g L ): Glucose, 20; Corn steep liquor, 15; NaNO 3, 1.0; KH2PO4, 6.0; MgSO4.7H20, 3.0; CuSO4.5H20, 0.002; FeSO4.7H20, 0.001; ZnSO4.7H20, 0.01 was then used for yeast biomass production in a pilot scale 16-L bioreactor in batch culture under controlled and uncontrolled pH. The highest b iomass of 8.2 g L -1 was achieved in pH controlled culture. This was almost double of the biomass obtained in shake fl sk. In conclusion, production process has been d eveloped for high cell mass production of the biotherapeutic yea st S. boulardii in semi-industrial scale.