Genorne polymorphisms and gene differential expression in a 'back-and-forth' ploidy-altered series of weeping lovegrass (Eragrostis curvula)
Genorne polymorphisms and gene differential expression in a 'back-and-forth' ploidy-altered series of weeping lovegrass (Eragrostis curvula)
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DOI：
10.1016/j.jplph.2006.07.002
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发表时间：
2007-08-01
影响因子：
4.3
通讯作者：
Pessino, Silvina C.
中科院分区：
文献类型：
--
作者：
Mecchia, Martin A.;Ochogavia, Ana;Pessino, Silvina C.
用分子标记分析了四倍体二倍体化后用秋水仙碱进行染色体再加倍获得的整倍体系列的基因组结构。在两个时期之间检测到相当水平的基因组多态性。奇怪的是，大量的分子标记在倍性的连续变化后表现出反向行为，这表明基因组的改变是特异性的，并赋予给定倍性水平的遗传结构特征。通过测序证实了真正的逆转。聚类分析表明，四倍体与二倍体的亲缘关系较远。多态可逆序列主要涉及非编码区，尽管其中一些序列与已知基因具有序列同源性。与p型腺苷三磷酸酶对应的反向序列在二倍体和colchiploid的cDNA文库中存在差异，但在原始四倍体中未发现表达。实时聚合酶链反应验证的花序转录组分析显示，无融合四倍体和有性二倍体植株之间存在0.34%的多态性带。有几个多态序列与已知的基因相对应。可能的相关性
Molecular markers were used to analyze the genomic structure of an euploid series of Eragrostis curvula, obtained after a tetraploid dihaploidization procedure followed by chromosome re-doubling with colchicine. Considerable Levels of genome polymorphisms were detected between tines. Curiously, a significant number of molecular markers showed a revertant behavior following the successive changes of ploidy, suggesting that genome alterations were specific and conferred genetic structures characteristic of a given ploidy Level. Genuine reversion was confirmed by sequencing. Cluster analysis demonstrated grouping of tetraploids white the diploid was more distantly related with respect to the rest of the plants. Polymorphic revertant sequences involved mostly non-coding regions, although some of them displayed sequence homology to known genes. A revertant sequence corresponding to a P-type adenosine triphosphatase was found to be differentially represented in cDNA Libraries obtained from the diploid and a colchiploid, but was not found expressed in the original tetraploid. Transcriptome profiling of inflorescence followed by real-time polymerase chain reaction validation showed 0.34% polymorphic bands between apomictic tetraploid and sexual diploid plants. Several of the polymorphic sequences corresponded to known genes. Possible correlation