Molecular cloning of a novel high molecular weight mucin (MG1) from human sublingual gland.
Molecular cloning of a novel high molecular weight mucin (MG1) from human sublingual gland.
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来自人类舌下腺的新型高分子量粘蛋白（MG1）的分子克隆。
DOI：
10.1006/bbrc.1995.2884
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Oppenheim,FG
中科院分区：
文献类型：
--
作者：
Troxler,RF;Offner,GD;Zhang,F;Iontcheva,I;Oppenheim,FG
用抗去糖基化MG 1的多克隆抗血清筛选人舌下腺cDNA文库，分离到一个阳性克隆pSM 2 -1，其编码该粘蛋白羧基端区域的196个氨基酸。该区域富含半胱氨酸，并且在末端羧基末端结构域上游含有C2样结构域，其中半胱氨酸的排列与人血管性血友病因子、人肠粘蛋白MUC 2、人气管支气管粘蛋白MUC 5以及猪和牛颌下腺粘蛋白中的半胱氨酸排列几乎相同。北方分析表明，MG 1在舌下腺中表达丰富，而在颌下腺中几乎检测不到。这项研究提供了第一个初级序列数据的人唾液粘蛋白MG 1和结果的意义进行了讨论，相对于MG 1的生物合成和差异表达的人唾液腺。
A human sublingual gland cDNA library was screened with a polyclonal antiserum against deglycosylated MG1 and a positive clone, pSM2-1, was isolated which codes for 196 amino acids in the carboxyl-terminal region of this mucin. This region is cysteine-rich and contains a C2-like domain upstream of the extreme carboxyl-terminal domain in which the arrangement of cysteines is nearly identical to that in human von Willebrand factor, human intestinal mucin MUC2, human tracheobronchial mucin MUC5 and porcine and bovine submaxillary gland mucins. Northern analyses with pSM2-1 showed MG1 transcripts are abundant in sublingual gland and barely detectable in submandibular gland. This study provides the first primary sequence data on human salivary mucin MG1 and the significance of the results is discussed with respect to the biosynthesis and differential expression of MG1 in human salivary glands.