Epstein-Barr Virus-associated Gastric Carcinoma: A Distinct Carcinoma of Gastric Phenotype by Claudin Expression Profiling
Epstein-Barr Virus-associated Gastric Carcinoma: A Distinct Carcinoma of Gastric Phenotype by Claudin Expression Profiling
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DOI：
10.1369/jhc.2009.953810
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发表时间：
2009-08-01
影响因子：
3.2
通讯作者：
Fukayama, Masashi
中科院分区：
文献类型：
--
作者：
Shinozaki, Aya;Ushiku, Tetsuo;Fukayama, Masashi
EB病毒相关性胃癌是一种具有特征性临床病理特征的独特亚型。为了更好地描述其细胞特征，我们对43例EBV相关性胃癌、68例EBV阴性胃癌以及成人和胎儿的非肿瘤性胃粘膜进行了免疫组化检查。我们定量了主要紧密连接蛋白claudin（CLDN）-1、-3、-4、-7和-18以及胃粘蛋白（MUC 5AC和MUC 6）、肠粘蛋白（MUC 2）和CD 10的表达。EBV相关的GC显示CLDN 18表达的高频率（84%）和CLDN 3表达的低频率（5%）。该表达谱与成人和胎儿正常胃上皮的表达谱一致。几乎一半的EBV相关胃癌病例显示胃粘蛋白表达，而另一半缺乏粘蛋白或CD 10表达。相反，如CLDN 3和CLDN 18的表达谱所示，EBV阴性GC包括四种不同CLDN表型的异质组：胃、肠、混合和具有可变粘蛋白表达模式的未分化类型。这些结果表明，EBV相关的GC是相当同质的细胞分化方面，它很好地保留了原始细胞的性质。EBV相关的GC可能经历不同的致癌过程，这与EBV阴性的GC不同。(C)Histochem Cytochem 57：775-785，2009）
Epstein-Barr virus (EBV)-associated gastric carcinoma (GC) is a distinct subtype with characteristic clinicopathological features. To better characterize its cellular characteristics, 43 cases of EBV-associated GC, 68 cases of EBV-negative GC, and non-neoplastic gastric mucosa in adults and fetuses were examined immunohistochemically. We quantified the expression of the major tight-junction protein claudin (CLDN) -1, -3, -4, -7, and -18 together with gastric mucins (MUC5AC and MUC6), intestinal mucin (MUC2), and CD10. EBV-associated GC showed a high frequency of CLDN18 expression (84%) and a low frequency of CLDN3 expression (5%). This expression profile corresponded to that of normal gastric epithelium in adults and fetuses. Almost half of the EBV-associated GC cases demonstrated gastric mucin expression, whereas the other half lacked mucin or CD10 expression. In contrast, as demonstrated by the expression profiles of CLDN3 and CLDN18, EBV-negative GC comprised a heterogeneous group of four different CLDN phenotypes: gastric, intestinal, mixed, and an undifferentiated type with variable expression patterns of mucins. These results indicate that EBV-associated GC is considerably homogenous with regard to cellular differentiation and that it preserves well the nature of the cells of origin. EBV-associated GC may undergo distinct carcinogenic processes, which differ from those of EBV-negative GC. (C) Histochem Cytochem 57:775-785, 2009)