UNIQUE AND OVERLAPPING POLLUTANT STRESS PROTEINS OF ESCHERICHIA-COLI
UNIQUE AND OVERLAPPING POLLUTANT STRESS PROTEINS OF ESCHERICHIA-COLI
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DOI：
10.1128/aem.58.1.331-334.1992
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发表时间：
1992-01-01
影响因子：
4.4
通讯作者：
MATIN, A
中科院分区：
文献类型：
--
作者：
BLOM, A;HARDER, W;MATIN, A
大肠杆菌的生长批培养物暴露于9种不同的“模型微污染物”（苯，氯化镉，毒死蜱，2，4-二氯苯胺，邻苯二甲酸二辛酯，六氯苯，五氯苯酚，三氯乙烯，和四丙基苯磺酸盐）导致13至39种蛋白质的诱导，通过二维凝胶电泳分析。其中一些蛋白质与热休克和碳饥饿蛋白重叠，但至少50%是特定化学物质所特有的。应激蛋白诱导表现出时间模式，表明顺序基因表达。即使在对生长没有影响的浓度下，也会发生化学应激蛋白质合成。因此，这些蛋白质的合成可以作为胁迫和环境污染性质的敏感指标。
Exposure of growing batch cultures of Escherichia coli to nine different "model micropollutants" (benzene, cadmium chloride, chlorpyrivos, 2,4-dichloroaniline, dioctylphtalate, hexachlorobenzene, pentachlorophenol, trichloroethylene, and tetrapropylbenzosulfonate) led to the induction of 13 to 39 proteins, as analyzed by two-dimensional gel electrophoresis. Some of these proteins overlapped with heat shock and carbon starvation proteins, but at least 50% were unique to a given chemical. The stress protein induction showed a temporal pattern, indicating sequential gene expression. Chemical stress protein synthesis occurred even at concentrations that had no effect on growth. Thus, the synthesis of these proteins can be a sensitive index of stress and the nature of environmental pollution.