ADP/ATP TRANSLOCATOR IS ESSENTIAL ONLY FOR ANAEROBIC GROWTH OF YEAST SACCHAROMYCES-CEREVISIAE
ADP/ATP TRANSLOCATOR IS ESSENTIAL ONLY FOR ANAEROBIC GROWTH OF YEAST SACCHAROMYCES-CEREVISIAE
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DOI：
10.1016/0014-5793(91)81059-h
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发表时间：
1991-09-09
期刊：
影响因子：
3.5
通讯作者：
KOLAROV, J
中科院分区：
文献类型：
--
作者：
DRGON, T;SABOVA, L;KOLAROV, J
通过基因破坏技术，编码线粒体 ADP/ATP 易位子的所有三个基因（AAC1、AAC2 和 AAC3）在单倍体酵母菌株中失活。三重突变体仍然能够在可发酵碳源上生长，但只有在氧气存在的情况下。在有氧条件下，在 AAC2 基因被破坏的所有突变体中均未检测到易位蛋白或载体介导的运输。进一步表明，功能性AAC基因产物仅对于酿酒酵母的厌氧生长是必需的，但对于去抑制条件下的生长不是必需的。在厌氧条件下，检测不到的 AAC3 基因产物足以确保细胞生长和增殖。
All three genes (AAC1,AAC2 and AAC3) encoding the mitochondrial ADP/ATP translocator, were inactivated in a haploid yeast strain by a gene disruption technique. The triple mutant was still able to grow on fermentable carbon sources but only in the presence of oxygen. Under aerobic conditions neither translocator-protein nor carrier-mediated transport was detected in all mutants in which the AAC2 gene was disrupted. It was further shown that a functional AAC genes product is essential only for anaerobic growth of Saccharomyces cerevisiae but not for growth under derepressed conditions. Under anaerobic conditions a non-detectable amount of AAC3 gene product is sufficient to ensure the cell growth and multiplication.