Effects of cAMP- and cGMP-dependent protein kinases, and calmodulin on Ca2+ uptake by highly purified sarcolemmal vesicles of vascular smooth muscle.
Effects of cAMP- and cGMP-dependent protein kinases, and calmodulin on Ca2+ uptake by highly purified sarcolemmal vesicles of vascular smooth muscle.
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cAMP 和 cGMP 依赖性蛋白激酶以及钙调蛋白对血管平滑肌高度纯化的肌膜囊泡摄取 Ca2+ 的影响。
DOI：
10.1016/0005-2736(84)90552-2
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
H. Kuriyama
中科院分区：
文献类型：
--
作者：
E. Suematsu;M. Hirata;H. Kuriyama
通过差速离心和蔗糖密度梯度离心从猪胸主动脉制备血管平滑肌的肌膜部分。在这些级分中，5′-核苷酸酶（一种假定的质膜标记酶）活性高，而鱼藤酮不敏感的 NADH 细胞色素还原酶（肌浆网标记酶）活性低。在这些级分中，Ca2+ 的吸收是 ATP 依赖性的。低浓度的皂苷抑制质膜对 Ca2+ 的吸收，但不抑制肌浆网的 Ca2+ 吸收，抑制了该部分 65% 的 Ca2+ 吸收。 cAMP 和 cGMP 依赖性蛋白激酶以及钙调蛋白增强了该级分的 Ca2+ 摄取。 cAMP 依赖性蛋白激酶增强 28 和 22 kDA 蛋白的磷酸化，而 cGMP 依赖性蛋白激酶则磷酸化 35 kDa 蛋白。 Ca2+ 和钙调蛋白可增强 100、75、65、41 和 22 kDa 蛋白质的磷酸化。这些结果表明，cAMP 和 cGMP 依赖性蛋白激酶以及钙调蛋白在肌膜中 Ca2+ 转运中发挥重要作用，并且磷酸化蛋白可能与肌膜中 Ca2+ 转运的增强有关。
Sarcolemmal fractions of vascular smooth muscles were prepared from porcine thoracic aortae by differential and sucrose density gradient centrifugation. In these fractions, there was a high activity of 5′-nucleotidase, a putative marker enzyme of plasma membrane, and a low activity of rotenone insensitive NADH-cytochromecreductase a marker of sarcoplasmic reticulum. In these fractions, the Ca2+uptake was ATP-dependent. A low concentration of saponin which inhibited Ca2+uptake by the plasma membrane but not by the sarcoplasmic reticulum, inhibited 65% of the Ca2+uptake of this fraction. The Ca2+uptake of this fraction was enhanced by cAMP- and cGMP-dependent protein kinases, and by calmodulin. The cAMP-dependent protein kinase enhanced the phosphorylation of 28 and 22 kDA proteins, while the cGMP-dependent protein kinase phosphorylated the 35 kDa protein. The phosphorylation of 100, 75, 65, 41 and 22 kDa proteins was enhanced by Ca2+and calmodulin. These results indicate that cAMP- and cGMP-dependent protein kinases as well as calmodulin play important roles in Ca2+transport in the sarcolemma, and that the phosphorylated proteins may be associated with an enhancement of Ca2+transport in the sarcolemma.
钙调蛋白敏感的 ATP 依赖性 Ca2+ 跨脂肪细胞质膜转运。
DOI：
--
发表时间：
1980
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Pershadsingh,HA;Landt,M;McDonald,JM
通讯作者：
McDonald,JM
胰岛细胞内质网和质膜主动 Ca2 转运特性的比较。
DOI：
10.1016/0005-2736(83)90483-2
发表时间：
1983
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Colca,JR;Kotagal,N;Lacy,PE;McDaniel,ML
通讯作者：
McDaniel,ML