Retrograde transport of horseradish peroxidase in transected axons. 3. Entry into injured axons and subsequent localization in perikaryon
Retrograde transport of horseradish peroxidase in transected axons. 3. Entry into injured axons and subsequent localization in perikaryon
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横切轴突中辣根过氧化物酶的逆行运输。
DOI：
10.1016/0006-8993(76)90507-2
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发表时间：
1976
期刊：
影响因子：
2.9
通讯作者：
Y. Olsson
中科院分区：
文献类型：
--
作者：
K. Kristensson;Y. Olsson
辣根过氧化物酶（HRP）应用于挤压的小鼠坐骨神经，通过受损的神经束膜扩散到神经内膜。在损伤区域，HRP进入受损的有髓和无髓轴突，形成反应产物柱，其向病变近端延伸数毫米。超微结构上，HRP粘附在轴浆的内表面和这样的柱的神经管和神经丝的表面。在更近端的水平轴突含有HRP的泡状和管状细胞器，后来，相应的脊神经节的神经细胞体显示HRP，积累在胞质囊泡，杯状体，多泡体和小管的无颗粒内质网。显着较少的HRP到达神经元的脊神经节时，施加到神经30或60分钟后的挤压。在这样的时间间隔后，也很少观察到含有HRP的轴突。可以想象，HRP在压碎后立即进入压碎的轴突，作为受损的细胞反应。随后，它被整合到轴突中更高的细胞器中，从那里逆行运输到核周体。这种外源性大分子突然非特异性流入轴突切断的神经元及其随后转运到核周体的现象可能与神经元对轴突损伤的某些生化和形态学反应（例如溶酶体改变）的发展有关。
Horseradish peroxidase (HRP) applied to crushed mouse sciatic nerves diffused through the damaged perineurium into the endoneurium. In the injured area, HRP passed into damaged myelinated and unmyelinated axons forming columns of reaction product, which extended for several millimeters proximally to the lesion. Ultrastructurally, HRP adhered to the inner surface of the axoplasm and to the surfaces of neurotubules and neurofilaments in such columns. At more proximal levels axons contained HRP in vesicular and tubular organelles and, later, nerve cell bodies of the corresponding spinal ganglia showed HRP, accumulation in cytoplasmic vesicles, cup-shaped bodies, multivesicular bodies and tubules of agranular endoplasmic reticulum. Markedly less HRP reached neurons in the spinal ganglia when applied to the nerve 30 or 60 min after the crush. After such time intervals solid HRP containing axons were also less frequently observed.Conceivably, HRP enters crushed axons momentarily after a crush as an injured cell reaction. Subsequently it is incorporated into organelles higher up in the axons, from where retrograde transport to the perikaryon will fellow. This phenomenon of a sudden non-specific influx of exogenous macromolecules into axotomized neurons and their subsequent transport to the perikaryon might be relevant for development of certain biochemical and morphological responses, e.g. lysosomal alterations, of the neuron to an axonal injury.