Synthesis of Malto-Oligosaccharides Via the Acceptor Reaction Catalyzed by Cyclodextrin Glycosyltransferases
Synthesis of Malto-Oligosaccharides Via the Acceptor Reaction Catalyzed by Cyclodextrin Glycosyltransferases
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DOI：
10.3109/10242420109103514
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发表时间：
2001-01
影响因子：
1.8
通讯作者：
M. T. Martín;M. Alcalde;F. Plou;L. Dijkhuizen;A. Ballesteros
中科院分区：
文献类型：
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作者：
M. T. Martín;M. Alcalde;F. Plou;L. Dijkhuizen;A. Ballesteros
热厌氧杆菌属环麦芽糖糊精糖基转移酶 (CGTase) 的歧化活性（分子间转糖基化）。对环状芽孢杆菌菌株251进行了研究。使用可溶性淀粉作为供体，来自Thermoanaerobacter sp.的CGTase。以所有作为受体测试的麦芽低聚糖显示出最高的转糖基活性。当淀粉：D-葡萄糖的比例为2:1至1:2（w/w）时，环糊精的形成被完全抑制，并产生了一系列同源麦芽低聚糖（Gn）。 48小时内淀粉转化为受体产物的转化率为63-79%。形成的麦芽低聚糖的聚合度可以通过所提供的淀粉：D-葡萄糖的比例来调节；当比例为 1:2 (w/w) 时，反应对于 G2-G3 的形成具有相当的选择性。
The disproportionation activity (intermolecular transglycosylation) of cyclomaltodextrin glycosyltransferases (CGTases) from Thermoanaerobacter sp. and Bacillus circulans strain 251 was studied. Using soluble starch as donor, the CGTase from Thermoanaerobacter sp. showed the highest transglycosylation activity with all the malto-oligosaccharides tested as acceptors. At ratios of starch: D-glucose from 2:1 to 1:2 (w/w), the formation of cyclodextrins was completely inhibited, and a homologous series of malto-oligosaccharides (Gn) was produced. The conversion of starch into acceptor products was in the range of 63-79% in 48 h. The degree of polymerisation of malto-oligosaccharides formed could be modulated by the ratio of starch: D-glucose provided; at a ratio of 1:2 (w/w), the reaction was quite selective for the formation of G2-G3.