Analysis of the yeast short-term Crabtree effect and its origin.
Analysis of the yeast short-term Crabtree effect and its origin.
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DOI：
10.1111/febs.13019
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发表时间：
2014-11
期刊：
影响因子：
--
通讯作者：
Piškur J
中科院分区：
文献类型：
--
作者：
Hagman A;Säll T;Piškur J
短期Crabtree效应被定义为响应于向糖限制的酵母培养物提供过量糖的脉冲而立即发生有氧酒精发酵。在这里，我们已经确定了10种酵母菌具有明确的系统发育关系。酵母菌种培养葡萄糖限制条件下，我们研究了他们的一般碳代谢葡萄糖脉冲。我们收集了大量关于葡萄糖和氧气消耗、乙醇和二氧化碳生成的数据。我们的结论是，毕赤酵母，德巴利酵母，假囊酵母和克鲁维酵母marxianus酵母没有表现出任何显着的乙醇形成，而乳酸克鲁维酵母的行为作为一个中间酵母，和Lachancea，Torulaspora，Vanderwaltozyma和酵母表现出快速乙醇积累。根据目前的数据和我们以前的数据有关的长期克拉布特里效应的存在超过40种酵母菌，我们推测，短期效应的起源可能与长期克拉布特里效应的起源在真菌目谱系，发生在1.5亿年前。
The short-term Crabtree effect is defined as the immediate occurrence of aerobic alcoholic fermentation in response to provision of a pulse of excess sugar to sugar-limited yeast cultures. Here we have characterized ten yeast species with a clearly defined phylogenetic relationship. Yeast species were cultivated under glucose-limited conditions, and we studied their general carbon metabolism in response to a glucose pulse. We generated an extensive collection of data on glucose and oxygen consumption, and ethanol and carbon dioxide generation. We conclude that the Pichia,Debaryomyces,Eremothecium and Kluyveromyces marxianus yeasts do not exhibit any significant ethanol formation, while Kluyveromyces lactis behaves as an intermediate yeast, and Lachancea,Torulaspora,Vanderwaltozyma and Saccharomyces yeasts exhibit rapid ethanol accumulation. Based on the present data and our previous data relating to the presence of the long-term Crabtree effect in over 40 yeast species, we speculate that the origin of the short-term effect may coincide with the origin of the long-term Crabtree effect in the Saccharomycetales lineage, occurring ∼ 150 million years ago.
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影响因子：
3.8
作者：
SONNLEITNER, B;KAPPELI, O
通讯作者：
KAPPELI, O
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作者：
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