Cucumber green mottle mosaic virus (CGMMV) can induce hair-like tissues on genus Cucumis seeds
Cucumber green mottle mosaic virus (CGMMV) can induce hair-like tissues on genus Cucumis seeds
复制标题
DOI：
10.1016/j.scienta.2012.08.016
复制
发表时间：
2012-10
影响因子：
4.3
通讯作者：
Seung-Yeol Lee;N. K. Win;Donghwi Cho;Su-Heon Lee;H. Jung
中科院分区：
文献类型：
--
作者：
Seung-Yeol Lee;N. K. Win;Donghwi Cho;Su-Heon Lee;H. Jung
黄瓜绿斑花叶病毒（CGMMV）侵染黄瓜属种子后，其表面发生了明显的形态变化。将染病种子和健康种子用乙醇系列处理后，在染病种子表面观察到不同寻常的毛发状组织。用立体显微镜和场发射扫描电镜研究了这些组织的形态。在黄瓜、甜瓜和甜瓜的种皮表面观察到毛状组织，并从种皮上凸起。同样处理的健康黄瓜和麝香瓜种皮上未发现这种组织，但在东方甜瓜种皮上发现了这种组织。不同种类黄瓜种子的毛状组织宽度不同，甜瓜为2 ~ 2.5μm，甜瓜和黄瓜种子为9 ~ 10μm。RT-PCR和巢式PCR再次证实，种皮呈毛状组织的种子感染了CGMMV。因此，本研究表明，毛状组织是CGMMV诱导的黄瓜属种子外部形态变化的一种独特症状。
Distinct morphological changes were observed on surface of Cucumber green mottle mosaic virus (CGMMV) infected genus Cucumis seeds. When the infected and healthy seeds were treated with ethanol series, unusual hair-like tissues were observed on surface of infected seeds. Morphology of these tissues was studied by stereo microscopy and field emission scanning electron microscopy. The hair-like tissues were observed on surface of seed coat of infected cucumber, musk melon and oriental melon and raised from the seed coats. Such tissues were not observed on seed coat of the same treated healthy cucumber and musk melon but such tissues were found as attached to seed coat of oriental melons. The width of hair-like tissues varied depend on species of genus Cucumis seeds, 2–2.5μm on musk melon and 9–10μm on oriental melon and cucumber seeds. The phenomenon was re-confirmed by RT-PCR and nested PCR assays that the seeds which had hair-like tissues on their seed coats were infected by CGMMV. Therefore, this study suggests that the hair-like tissues are a unique symptom as external morphological changes of genus Cucumis seeds induced by CGMMV.