Enhanced microbial degradation of pentachlorophenol from soil in the presence of earthworms: Evidence of functional bacteria using DNA-stable isotope probing
Enhanced microbial degradation of pentachlorophenol from soil in the presence of earthworms: Evidence of functional bacteria using DNA-stable isotope probing
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DOI：
10.1016/j.soilbio.2014.11.011
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发表时间：
2015-02
影响因子：
9.7
通讯作者：
Xiaomin Li;Zhong Lin;Chunling Luo;Jing Bai;Yingtao Sun;Yongtao Li
中科院分区：
文献类型：
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作者：
Xiaomin Li;Zhong Lin;Chunling Luo;Jing Bai;Yingtao Sun;Yongtao Li
研究了两种植物（AmynthasrobustusPerrier和EschereniafetidaSavigny）对土壤微生物降解五氯酚（PCP）的影响。五氯酚降解微生物鉴定使用DNA稳定同位素探针（SIP）。结果表明，在不加任何改良剂的情况下，土壤颗粒和有机组分对五氯酚的吸附和固定是其在土壤中的主要归宿。两种植物的接种显着提高土壤PCP的消失和基础呼吸。DNA-SIP结果表明，克雷伯氏菌属、嗜铜菌属、气单胞菌属和伯克霍尔德氏菌属4种细菌。结果表明，在粗壮放线菌存在的情况下，[13 C]标记PCP处理土壤微宇宙中的Cupriavidus和Aeromonassp.在接种前的肠道中也检测到了，它们的相对丰度受到蚯蚓的影响。这些结果表明，蚯蚓可以将功能菌引入土壤，增加PCP降解菌的数量，从而加速土壤PCP的降解。
This study investigated the effect of two earthworm species (AmynthasrobustusPerrier andEisenia fetidaSavigny) on the soil microbial degradation of pentachlorophenol (PCP). PCP-degrading microbes were identified using DNA-stable isotope probing (SIP). The results showed that adsorption and fixing to soil particles and organic fractions dominated the fate of PCP in soil without any amendments. The inoculation of both earthworm species significantly enhanced soil PCP disappearance and basal respiration. The DNA-SIP results revealed thatKlebsiella,Cupriavidus,Aeromonas, andBurkholderiaspp. were present at higher relative abundances in [13C]-labeled-PCP-amended soil microcosms than [12C]-PCP-amended soil in the presence ofA. robustus, indicating that these bacterial species were responsible for PCP assimilation.CupriavidusandAeromonassp. were also detected in the earthworm gut before inoculation, and their relative abundance was affected by earthworms. These results demonstrated that earthworms can introduce functional bacteria into soils and increase the population of PCP-degrading bacteria, thereby accelerating soil PCP degradation.