Resistant starch from high amylose maize (HAM-RS2) reduces body fat and increases gut bacteria in ovariectomized (OVX) rats.
Resistant starch from high amylose maize (HAM-RS2) reduces body fat and increases gut bacteria in ovariectomized (OVX) rats.
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DOI：
10.1002/oby.20109
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发表时间：
2013-05
期刊：
影响因子：
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通讯作者：
Senevirathne RN
中科院分区：
文献类型：
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作者：
Keenan MJ;Janes M;Robert J;Martin RJ;Raggio AM;McCutcheon KL;Pelkman C;Tulley R;Goita M;Durham HA;Zhou J;Senevirathne RN
绝经后肥胖是老年女性的一个健康问题。在这种情况下，微生物群可能会发生变化。用益生元改变微生物群是改善更年期女性健康的一种可行策略。在一项 2 × 2 析因研究中，来自高直链淀粉玉米的 2 型抗性淀粉 (HAM-RS2) 被用作大鼠的益生元，其中两个水平的 HAM-RS2（饮食重量的 0 % 或 29.7%）分别称为能量控制 (EC) 和 HAM-RS2 饮食；以及两个级别的手术：卵巢切除术（OVX）和假手术。在 6 周的术后恢复期内，OVX 大鼠在消耗相似数量的食物的情况下体重增加了更多。随后，与 EC 饮食相比，食用 HAM-RS2 导致 OVX 和假大鼠腹部脂肪减少；但当对去内脏体重（体重减去胃肠道）进行标准化时，手术没有效果，仅通过 HAM-RS2 减少。估计已知会发酵 HAM-RS2 或代谢初始发酵产物的目标细菌种群。 OVX 和假手术大鼠的饮食中 HAM-RS2 的所有细菌的细菌水平均有所增加。此外，培养技术和 qPCR 也提供了类似的结果。这项研究表明，正如预期的那样，OVX 会增加肥胖。然而，与之前在肥胖小鼠中观察到的效果相反，这并没有阻止 HAM-RS2 的发酵，因此与 OVX 相关的脂肪增加减弱。
Obesity after menopause is a health concern for older females. Changes in the microbiota are likely to occur with this condition. Modifying the microbiota with a prebiotic is a plausible strategy for improving the health of menopausal females. Resistant starch type 2 from high-amylose maize (HAM-RS2) was used as a prebiotic in rats in a 2 × 2 factorial study with two levels of HAM-RS2 (0 or 29.7% of weight of diet) referred to as energy control (EC) and HAM-RS2 diets, respectively; and two levels of surgery, ovariectomized (OVX) and sham. In a 6-week, postsurgery recovery period, OVX rats gained more body weight with consumption of a similar amount of food. Subsequently, consumption of HAM-RS2 versus EC diets resulted in reduced abdominal fat in both OVX and sham rats; but when normalized for disemboweled body weight (body weight minus GI tract), there was no effect of surgery, only reduction with HAM-RS2. Targeted bacterial populations were estimated that are known to ferment HAM-RS2 or metabolize the products of that initial fermentation. OVX and sham rats demonstrated increased bacterial levels with dietary HAM-RS2 for all bacteria. Additionally, culture techniques and qPCR provided similar results. This study shows that, as expected, OVX increases adiposity. However, contrary to previous effects seen in obese mice, this did not prevent fermentation of HAM-RS2 and consequently, the fat gain associated with OVX was attenuated.