Nano-structured polymers enhance bladder smooth muscle cell function
Nano-structured polymers enhance bladder smooth muscle cell function
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DOI：
10.1016/s0142-9612(03)00123-6
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发表时间：
2003-08-01
期刊：
影响因子：
14
通讯作者：
Haberstroh, KM
中科院分区：
文献类型：
--
作者：
Thapa, A;Miller, DC;Haberstroh, KM
本研究的假设是，模拟天然膀胱组织的纳米形貌的生物相容性材料将增强细胞反应并导致更好的体内组织整合。先前的体外研究已经验证了通过化学蚀刻程序成功地将聚（乳酸-共-乙醇酸）（PLGA）和聚（醚氨基甲酸酯）（PU）膜的表面特征尺寸减小到纳米范围的能力。这些研究的结果还提供了第一个证据，即膀胱平滑肌细胞粘附增强化学处理的纳米结构的聚合物表面相比，其传统的同行。虽然细胞粘附对于生物材料的成功是必要的，但随后的细胞功能（例如长期细胞生长和增殖）对于组织向内生长和长期植入物存活也是至关重要的。因此，目前的体外研究。研究了膀胱平滑肌细胞在这些新型纳米结构聚合物上在1、3和5天的扩展期内的功能。结果表明，细胞数量的影响，表面粗糙度和表面化学变化：重要的贡献者，但是，增加纳米表面粗糙度。这一说法得到了以下事实的支持：一旦使用铸造技术消除化学变化，与传统的PLGA和PU相比，纳米结构的细胞数量就会增加。(C)2003爱思唯尔科技有限公司版权所有。
It is the hypothesis of the present study that a biocompatible material which mimics the nanometer topography of native bladder tissue will enhance cellular responses and lead to better tissue integration in vivo. Previous in vitro studies have verified the ability to successfully reduce the surface feature dimensions of poly(lactic-co-glycolic acid) (PLGA) and poly(ether urethane) (PU) films into the nanometer regime via chemical etching procedures. Results from these studies also provided the first evidence that bladder smooth muscle cell adhesion was enhanced on chemically treated nano-structured polymeric surfaces compared to their conventional counterparts. Although cell adhesion is necessary for a biomaterial's success, subsequent cell functions (such as long-term cell growth and proliferation) are also critical for tissue ingrowth and long-term implant survival. The present in vitro study, therefore. investigated the function of bladder smooth muscle cells on these novel, nano-structured polymers over the expanded periods of 1, 3 and 5 days. Results indicated that cell number was influenced by both surface roughness and surface chemistry changes: the important contributor, however, was increased nanometer surface roughness. This claim is supported by the fact that cell number was enhanced on nano-structured compared to conventional PLGA and PU once chemistry changes were eliminated using casting techniques. (C) 2003 Elsevier Science Ltd. All rights reserved.