The production of anti-inflammmatory cytockines in whole blood by physico-chemical induction
The production of anti-inflammmatory cytockines in whole blood by physico-chemical induction
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DOI：
10.1007/s00011-003-1197-1
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发表时间：
2003-10-01
影响因子：
6.7
通讯作者：
Wehling, P
中科院分区：
文献类型：
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作者：
Meijer, H;Reinecke, J;Wehling, P
目标和设计：细胞因子如白细胞介素-1（IL-1）在退行性肌肉骨骼疾病（包括骨关节炎）和多种炎症性疾病中起重要作用。抑制这类细胞因子作用的药剂在这类疾病中具有高治疗潜力。在这里，我们描述了一种新的方法，用于提高生产的白细胞介素-1受体拮抗剂（IL-1 Ra）和其他抗炎cytokines in whole blood.Material和方法：人静脉血中孵育的存在下，硫酸铬处理的玻璃珠。结果：玻璃微珠表面与全血细胞相互作用后，IL-1 Ra和IL-1 Ra相关细胞因子的产生增加。去除珠粒并离心产生富含TNF细胞因子的血清制剂。这种制剂是在治疗各种炎症和退行性disorder.Conclusions的治疗价值：增加从头生产的α细胞因子的直接物理化学诱导的全血中的Orthokin系统是可行的，并提供了一种替代的，新的方法来治疗轻度至中度OA和其他骨科疾病，如退行性脊柱疾病。
Objective and design: Cytokines such as interleukin-1 (IL-1) play an important role in degenerative musculo-skeletal diseases, including osteoarthritis, and a multitude of inflammatory disorders. Agents that inhibit the action of such cytokines have a high therapeutic potential in such diseases. Here we describe a new method for enhancing the production of the interleukin-1 receptor antagonist (IL-1Ra) and other anti-inflammatory cytokines in whole blood.Material and methods: Human venous blood was incubated in the presence of CrSO4-treated glass beads. Serum was recovered and the concentrations of IL-1Ra and other relevant cytokines were measured by ELISA.Results: The interaction of the glass bead surface with cells in whole blood increased production of IL-1Ra and antiinflammatory cytokines. Removal of the beads and centrifugation generated a serum preparation enriched in antiinflammatory cytokines. This preparation is of therapeutic value in treating various inflammatory and degenerative disorders.Conclusions: The increased de novo production of antiinflammatory cytokines by a direct physico-chemical induction of whole blood in the Orthokin system is feasible and offers an alternative, novel approach to treating mild to moderate OA and other orthopaedic conditions such as degenerative spine diseases.