Isolation, characterization and comparative studies of the N‐terminal peptides from soluble pig skin collagen
Isolation, characterization and comparative studies of the N‐terminal peptides from soluble pig skin collagen
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可溶性猪皮胶原蛋白 N 端肽的分离、表征和比较研究
DOI：
10.1016/0014-5793(74)80047-5
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发表时间：
1974
期刊：
影响因子：
3.5
通讯作者：
E. Heidemann
中科院分区：
文献类型：
--
作者：
S. Allam;E. Heidemann
胶原蛋白是一种普遍存在的结构蛋白，约占哺乳动物体内所有蛋白质重量的25%-30%。它在脊椎动物组织中起着重要的支撑作用。用溴化氰裂解甲硫基残基蛋白质链的方法，从不同来源的大鼠皮肤[L，2]、鸡皮[3]、小牛皮肤[4，5]和人皮肤胶原[6]中分离和鉴定了多种胶原。猪皮胶原蛋白由于其较差的或非抗原性的反应性，从其在医疗领域的适用性[7，11，12]的角度来看似乎很有趣。我们以前报道过从猪皮胶原蛋白的油链和CW2链中分离和鉴定大的CB多肽[8]。本文报道了可溶性猪皮胶原N-末端CB多肽的分离、鉴定及其与大鼠皮肤、小牛皮肤和人皮肤胶原多肽的比较。
Collagen is an ubiquitous structural protein, which constitutes about 25-30% by weight of all proteins in the mammalian body. It functions as an important supporting element in vertebrate tissues. Using the method of cleaving the protein chains at the methionyl residues with cyanogen bromide (CNBr), many species of collagen from different sources have been isolated and characterized, eg rat skin [l, 2], chick skin [3], calf skin [4, 5] and human skin collagen [6]. Pig skin collagen seems to be interesting from the point of view, that is, its applicability in the medical sector [7, 11, 12], due to its poor or non-antigenic reactivity. We reported previously about the isolation and characterization of the large CB-peptides derived from both oil-and cw2-chain of the pig skin collagen [8]. This report deals with the isolation, characterization and comparison of the N-terminal CB-peptides of soluble pig skin collagen with the corresponding peptides derived from rat skin, calf skin and human skin collagen.