Quantitative imaging of lymph function
Quantitative imaging of lymph function
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DOI：
10.1152/ajpheart.01223.2006
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发表时间：
2007-06-01
影响因子：
4.8
通讯作者：
Sevick-Muraca, Eva M.
中科院分区：
文献类型：
--
作者：
Sharma, Ruchi;Wang, Wei;Sevick-Muraca, Eva M.
在麻醉的腹部和后肢前部显示功能性淋巴显像。在皮内施用100-200 μ l 32 μ M吲哚菁绿色（ICG）和64 μ M透明质酸NIR成像缀合物以靶向淋巴内皮上的淋巴血管内皮受体（LYVE）-1之后，通过使用近红外（NIR）荧光成像，对完整的约克郡猪进行研究。NIR荧光成像采用780 nm激发光（类似于2 nW/cm（2））的照射和从ima成像剂产生的830 nm荧光的收集。我们的研究结果显示了成像的能力，立即贩运，ICG从丛，通过血管和淋巴管，并到浅表乳房。髂下动脉和髂中淋巴结其位于组织表面下方3cm处。ICG经淋巴管的“包”，长度为2-16 cm，以0.5-3.3脉冲/min的频率和0.23-0.75 cm/s的速度推进。淋巴推进与呼吸速率无关。在透明质酸显像剂的情况下。当染料立即通过神经丛并染色淋巴管和淋巴结时，不存在淋巴推进。淋巴成像分别需要5.0和11.9 μ g ICG和透明质酸结合物。我们的研究结果表明，微量的近红外光学成像剂和它们的共轭物有可能成像淋巴功能的患者患有淋巴相关疾病。
Functional lymphatic imaging, was demonstrated in the abdomen and anterior hindlimb of anesthetized. intact Yorkshire swine by using near-infrared (NIR) fluorescence imaging following intradermal administration of 100-200 mu l of 32 mu M indocyanine green (ICG) and 64 mu M hyaluronan NIR imaging conjugate to target the lymph vacular endothelial receptor (LYVE)-1 on the lymph endothelium. NIR fluorescence imaging employed illumination of 780 nm excitation light (similar to 2 nW/cm(2)) and collection of 830 nm fluorescence generated from the ima imaging agents. Our results show the ability to image the immediate trafficking, of ICG from the plexus, through the vessels and lymph-angions, and to the superficial mammary. subiliac. and middle iliac lymph nodes. which were located as (Jeep as 3 cin beneath the tissue Surface. "Packets" of ICG-transited lymph vessels of 2-16 cin length propelled at frequencies of 0.5-3.3 pulses/min and velocities of 0.23-0.75 cm/s. Lymph propulsion Was independent of respiration rate. In the case of the hyaluronan imaging agent. lymph propulsion was absent as the dye progressed immediately through the plexus and stained the lymph vessels and nodes. Lymph imaging required 5.0 and 11.9 mu g of ICG and hyaluronan conjugate, respectively. Our results Suggest that mnicrogram quantities of NIR optical imaging agents and their conjugates have a potential to image lymph function in patients suffering from lymph-related disorders.