A homologue of the Agrobacterium tumefaciens VirB and Bordetella pertussis Ptl type IV secretion systems is essential for intracellular survival of Brucella suis
A homologue of the Agrobacterium tumefaciens VirB and Bordetella pertussis Ptl type IV secretion systems is essential for intracellular survival of Brucella suis
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DOI：
10.1046/j.1365-2958.1999.01569.x
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发表时间：
1999-09-01
影响因子：
3.6
通讯作者：
Ramuz, M
中科院分区：
文献类型：
--
作者：
O'Callaghan, D;Cazevieille, C;Ramuz, M
分析猪布鲁氏杆菌1330的TnblaM突变体，鉴定为不能在Hela细胞中繁殖，使我们能够鉴定B的11860 bp区域。猪基因组编码IV型分泌系统，与根癌农杆菌的VirB系统和百日咳博德特氏菌的Ptl系统同源。DNA序列揭示了12个开放阅读框（ORF），其编码存在于具有类似遗传组织的农杆菌的pTi质粒中的11种VirB蛋白的同源物，以及第十二个ORF，其编码推定的脂蛋白，其与在细菌接合期间参与交配对形成的蛋白同源，并且与假单胞菌属物种用于结合到植物根部的粘附素同源。基于VirB4和VirB9蛋白质同源物序列的系统发育树表明，从DNA转移到蛋白质分泌的系统进化并不源于单一事件，而是蛋白质分泌系统独立进化。在人巨噬细胞的体外感染模型中，virB5、virB9或virB10中的四个独立突变体被高度减毒。通过与含有完整virB区域的质粒互补来恢复毒力。virB区似乎是B细胞内存活和增殖所必需的。猪。
Analysis of a TnblaM mutant of Brucella suis 1330, identified as being unable to multiply in Hela cells, allowed us to identify a 11860 bp region of the B. suis genome encoding a type IV secretion system, homologous to the VirB system of Agrobacterium tumefaciens and the Ptl system of Bordetella pertussis. DNA sequence revealed 12 open reading frames (ORFs) encoding homologues of the 11 VirB proteins present in the pTi plasmid of Agrobacterium with a similar genetic organization, and a twelfth ORF encoding a putative lipoprotein, homologous to a protein involved in mating pair formation during bacterial conjugation and to adhesins used by Pseudomonas species to bind to plant roots. Phylogenetic trees based on the sequences of VirB4 and VirB9 protein homologues suggest that evolution of the systems from DNA transfer towards protein secretion did not stem from a single event but that the protein secretion systems have evolved independently. Four independent mutants in virB5, virB9 or virB10 were highly attenuated in an in vitro infection model with human macrophages. The virulence was restored by complementation with a plasmid containing the full virB region. The virB region appears to be essential for the intracellular survival and multiplication of B. suis.