Interactions of airway smooth muscle cells with their tissue matrix: implications for contraction.
Interactions of airway smooth muscle cells with their tissue matrix: implications for contraction.
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DOI：
10.1513/pats.200704-048vs
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发表时间：
2012-12
期刊：
影响因子：
--
通讯作者：
Wenwu Zhang;S. Gunst
中科院分区：
文献类型：
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作者：
Wenwu Zhang;S. Gunst
气道平滑肌响应于机械振荡和拉伸而改变其刚度和收缩性的能力对于呼吸期间正常气道反应性的调节至关重要。气道平滑肌的机械适应特性被认为是由肌动球蛋白相互作用之外的动态细胞骨架过程引起的。肌动球蛋白相互作用和横桥循环被视为细胞收缩过程中发生的复杂和综合的细胞骨架事件的组成部分。这些事件是由大分子蛋白质复合物协调的，所述大分子蛋白质复合物与肌细胞和细胞外基质之间的粘附连接处的整合素蛋白质的胞质结构域相关联。根据这种模式，这些协调的细胞骨架事件是气道平滑肌中主动张力产生过程的重要组成部分，并且还用于使平滑肌细胞的形状和刚度适应其环境。收缩刺激引发气道平滑肌细胞膜下皮质内的肌动蛋白聚合，其可用于确定细胞形状并加强膜。结构蛋白如α-辅肌动蛋白向粘附连接的募集增强了膜粘附连接和肌动蛋白丝之间的连接强度。这些过程创造了一个强大而刚性的细胞骨架框架，用于传递由肌球蛋白和肌动蛋白丝相互作用产生的力。这一气道平滑肌功能调节模型为解释哮喘气道的正常生理行为和气道的病理生理特性提供了新的视角。
The ability of airway smooth muscle to alter its stiffness and contractility in response to mechanical oscillation and stretch is critically important for the regulation of normal airway responsiveness during breathing. The properties of mechanical adaptation in airway smooth muscle are proposed to result from dynamic cytoskeletal processes outside of the actomyosin interaction. The actomyosin interaction and crossbridge cycling are viewed as components of a complex and integrated array of cytoskeletal events that occur during cell contraction. These events are orchestrated by macromolecular protein complexes that associate with the cytoplasmic domains of integrin proteins at the adhesion junctions between muscle cells and the extracellular matrix. According to this paradigm, these concerted cytoskeletal events are essential components of the process of active tension generation in airway smooth muscle, and also serve to adapt the shape and stiffness of the smooth muscle cell to its environment. Contractile stimuli initiate actin polymerization within the submembranous cortex of the airway smooth muscle cell that may serve to determine the cells shape and strengthen the membrane. The recruitment of structural proteins such as alpha-actinin to adhesion junctions fortifies the strength of the connections between membrane adhesion junctions and actin filaments. These processes create a strong and rigid cytoskeletal framework for the transmission of force generated by the interaction of myosin and actin filaments. This model for the regulation of airway smooth muscle function can provide novel perspectives to explain the normal physiologic behavior of the airways and pathophysiologic properties of the airways in asthma.