Intracellular recording of in situ ventricular cells during ventricular fibrillation.
Intracellular recording of in situ ventricular cells during ventricular fibrillation.
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心室颤动期间原位心室细胞的细胞内记录。
DOI：
10.1152/ajpheart.1981.240.4.h465
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Akiyama,T
中科院分区：
文献类型：
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作者：
Akiyama,T
在室颤(VF)过程中，心肌细胞的跨膜动作电位(AP)尚未得到系统的研究。我们最近开发了一种运动补偿的微吸管支架，它可以支撑微吸管，并与心脏运动同步运动。在14只开胸麻醉犬，在阻断左前降支(LAD)前和再灌注室颤(VF)前10分钟记录心外膜下心肌细胞AP。在控制期间，AP的特征(平均+/-SE)为：兴奋率(R)，112+/-4次/分；静息电位(VR)，-79.3+/-1.0 mV；超射电位(VOV)，17.9+/-1.8 mV；90%AP时程(Apd)，251+/-8ms。VF时，再灌注区AP分别为：R，173+/-27次/分，VR，-60.6+/-2.2 mV，VOV，3.4+/-2.9 mV，APD134+/-9ms(P<0.001，除R外，与对照组比较均有显著差异)。在VF期间，维拉帕米使再灌注区的细胞不兴奋，但河豚毒素不使之兴奋。综上所述，在室性心动过速时，从心脏原位准确记录AP是可能的，并且再灌注区的心外膜下细胞似乎具有慢通道类型的AP。
Transmembrane action potentials (AP) from ventricular cells during ventricular fibrillation (VF) have not been systematically studied. We have recently developed a motion-compensated micropipette holder that holds a micropipette and moves in synchrony with heart motion. AP of subepicardial ventricular cells were recorded in 14 open-chest anesthetized dogs prior to occlusion (control) of left anterior descending artery (LAD) and during first 10 min of reperfusion-induced VF. During control, characteristics (means +/- SE) of AP are: excitation rate (R), 112 +/- 4 beats/min; resting potential (Vr), -79.3 +/- 1.0 mV; overshoot potential (Vov), 17.9 +/- 1.8 mV; 90% AP duration (APD), 251 +/- 8 ms. During VF, AP from the reperfused areas are: R, 173 +/- 27 beats/min; Vr, -60.6 +/- 2.2 mV; Vov, 3.4 +/- 2.9 mV; APD, 134 +/- 9 ms (significantly different from control at P less than 0.001, except R). During VF, cells from the reperfused areas are made inexcitable with verapamil, but not by tetrodotoxin. In conclusion, during VF accurate AP recording is possible from in situ heart, and subepicardial cells in reperfused areas seem to have AP of slow-channel type.