Identification of insulin-like growth factor-II in human seminal and follicular fluids.
Identification of insulin-like growth factor-II in human seminal and follicular fluids.
复制标题
人精液和卵泡液中胰岛素样生长因子-II 的鉴定。
DOI：
10.1016/0006-291x(86)90765-5
复制
发表时间：
1986
影响因子：
3.1
通讯作者：
Li,CH
中科院分区：
文献类型：
--
作者：
Ramasharma,K;Cabrera,CM;Li,CH
使用兔子体内产生的抗合成胰岛素样生长因子-II (IGF-II) 的抗血清来开发针对 IGF-II 的特异性放射免疫测定法 (RIA)。亲和纯化的抗体与 IGF-I 有 6% 的交叉反应性，但即使在 10 μg/管时也无法识别胰岛素。利用该 RIA 系统，在人卵泡液和精浆的混合样本中鉴定出了免疫反应性 IGF-II。人精液和卵泡液的酸-乙醇沉淀物在Sephadex G-50柱上进行色谱分离，IGF-II免疫反应级分进行反相高效液相色谱分析。发现免疫活性IGF-II在与合成IGF-II相同的位置被洗脱。该数据首次表明人类精浆和卵泡液中含有大量的 IGF-II。
Antisera raised in rabbits against synthetic insulin-like growth factor-II (IGF-II) were used to develop a specific radioimmunoassay (RIA) for IGF-II. Affinity purified antibodies showed 6% cross-reactivity with IGF-I but failed to recognize insulin even at 10 μg/tube. Utilizing this RIA system, immunoreactive IGF-II was identified in the pooled samples of human follicular fluid and seminal plasma. The acid-ethanol precipitates of human seminal and follicular fluids were chromatographed on Sephadex G-50 column and the IGF-II immunoreactive fractions were subjected to reversed-phase high performance liquid chromatography. It was found that immunoactive IGF-II was eluted in the same location as that of synthetic IGF-II. The data indicate for the first time that human seminal plasma and follicular fluid contain significant amounts of IGF-II.