Magnetic resonance imaging of haemorrhage within reperfused myocardial infarcts: possible interference with iron oxide-labelled cell tracking?
Magnetic resonance imaging of haemorrhage within reperfused myocardial infarcts: possible interference with iron oxide-labelled cell tracking?
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DOI：
10.1093/eurheartj/ehl059
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发表时间：
2006-07-01
影响因子：
39.3
通讯作者：
Wielopolski, Piotr A.
中科院分区：
文献类型：
--
作者：
van den Bos, Ewout J.;Baks, Timo;Wielopolski, Piotr A.
目的 磁共振成像 (MRI) 已被提议作为追踪心肌梗塞 (MI) 内氧化铁标记细胞的工具。然而，梗塞再灌注会加重微血管阻塞（MO）并导致出血。我们假设出血性心肌梗死会导致磁化率诱导的信号空洞，这可能会干扰氧化铁标记的细胞检测。 方法和结果 猪（n = 23）接受了 2 小时的左回旋动脉闭塞。在第 1 周和第 5 周进行电影、T2* 加权、灌注和延迟增强 MRI 扫描，然后进行离体高分辨率扫描。第 1 周时，21 只动物中有 17 只观察到 MO。八只动物中的五只在 T2* 加权扫描中观察到信号空洞，占梗塞面积的 24 +/- 22%。 MO 面积和信号空洞之间存在线性相关性（R-2 = 0.87；P = 0.002）。第 5 周时，13 只动物中的 2 只观察到 MO。在七只动物中发现了三只信号空洞。经组织学证实，离体扫描显示，由于存在出血，所有动物的 T2* 加权扫描均出现信号缺失。信号空洞干扰离体氧化铁标记细胞的检测（n = 21 次注射）。结论 再灌注 MI 中的出血会产生 MRI 信号空洞，这可能会妨碍氧化铁标记细胞的追踪。
Aims Magnetic resonance imaging (MRI) has been proposed as a tool to track iron oxide-labelled cells within myocardial infarction (MI). However, infarct reperfusion aggravates microvascular obstruction (MO) and causes haemorrhage. We hypothesized that haemorrhagic MI causes magnetic susceptibility-induced signal voids that may interfere with iron oxide-labelled cell detection.Methods and results Pigs (n = 23) underwent 2 h occlusion of the left circumflex artery. Cine, T2*-weighted, perfusion, and delayed enhancement MRI scans were performed at 1 and 5 weeks, followed by ex vivo high-resolution scanning. At 1 week, MO was observed in 17 out of 21 animals. Signal voids were observed on T2*-weighted scans in five out of eight animals, comprising 24 +/- 22% of the infarct area. A linear correlation was found between area of MO and signal voids (R-2 = 0.87; P = 0.002). At 5 weeks, MO was observed in two out of 13 animals. Signal voids were identified in three out of seven animals. Ex vivo scanning showed signal voids on T2*-weighted scanning in all animals because of the presence of haemorrhage, as confirmed by histology. Signal voids interfered with the detection of iron oxide-labelled cells ex vivo (n = 21 injections).Conclusion Haemorrhage in reperfused MI produces MRI signal voids, which may hamper tracking of iron oxide-labelled cells.