Routine measurement of calcium, magnesium, copper, zinc, and iron in urine and serum by inductively coupled plasma emission spectroscopy.
Routine measurement of calcium, magnesium, copper, zinc, and iron in urine and serum by inductively coupled plasma emission spectroscopy.
复制标题
通过电感耦合等离子体发射光谱法常规测量尿液和血清中的钙、镁、铜、锌和铁。
作者：
D. E. Nixon;T. Moyer;P. Johnson;J. McCall;A. Ness;W. Fjerstad;M. Wehde
我们描述了一种电感耦合等离子体原子发射光谱仪，该光谱仪适用于对血清或尿液中的钙、镁、铜、锌和铁进行常规、同步、直接分析，无需样品消化或预处理。该系统由廉价且容易获得的组件构成，可以分析 1 mL 或更小的样品。结果与标准原子吸收技术得出的结果几乎完全相关（r = 0.98 至 0.997）。使用来自不同来源的经认证的血清和尿液样本，我们证明该仪器可产生准确的结果，其精度优于认证值。该仪器对所有测试元素的血清或尿液中低于正常范围下限的一个数量级敏感，并对高于正常范围上限两个数量级的浓度做出线性响应。利用此处描述的系统，可以用与通过原子吸收分析单个元素所需的技术工作相同或更少的技术工作来分析这五种元素。
We describe an inductively coupled plasma atomic emission spectrometer that has been adapted to perform routine, simultaneous, direct analyses of calcium, magnesium, copper, zinc, and iron in serum or urine without sample digestion or pretreatment. The system, constructed with inexpensive, readily available components, can analyze 1-mL or smaller samples. Results correlate nearly perfectly with those derived by standard atomic absorption techniques (r = 0.98 to 0.997). Using certified serum and urine samples from various sources, we demonstrate that the instrument yields accurate results with a precision better than certified values. The instrument is sensitive to one order of magnitude less than the lower limit of the normal range in serum or urine for all elements tested, and responds linearly to concentrations two orders of magnitude higher than the upper limit of the normal range. With the system described here, these five elements can be assayed with the same or less technical effort than needed for a single element by atomic absorption.