Elevated expression of microRNA-30b in osteoarthritis and its role in ERG regulation of chondrocyte
Elevated expression of microRNA-30b in osteoarthritis and its role in ERG regulation of chondrocyte
复制标题
骨关节炎中 microRNA-30b 的表达升高及其在软骨细胞 ERG 调节中的作用
DOI：
10.1016/j.biopha.2015.10.014
复制
发表时间：
2015-12-01
影响因子：
7.5
通讯作者：
Zhang, Yukun
中科院分区：
文献类型：
--
作者：
Li, Lisong;Yang, Cao;Zhang, Yukun
ERG (ETS-related gene) belongs to the ETS family of transcription factors, and has been recently reported to contribute to homeostatic balance in skeleton cell plasticity. MicroRNA-30 (miR-30) family is also demonstrated to play a role in controlling chondrocyte differentiation. The current study investigated the miR-30b and ERG expression in articular cartilage of osteoarthritis (OA) patients. A total of 20 subjects, with 10 OA patients and 10 healthy participants, were included in this study. Human chondrosarcoma cell line SW1353 was used to explore the relationship of miR-30b and ERG in vitro. In OA patients, a significant increase of miR-30b and a decrease of ERG were observed in articular cartilage compared with Normal ones. MiR-30b mimic down-regulated the ERG mRNA and protein expression levels, while miR-30b inhibitor up-regulated ERG expression. In addition, miR-30b mimic also decreased the mRNA expression of COL2a and aggrecan, while miR-30b inhibitor had the opposite effect. Luciferase reporter assay confirmed that miR-30b targeted ERG. In conclusion, miR-30b was involved in the process of OA, and it probably functioned through its target gene ERG. (C) 2015 Elsevier Masson SAS. All rights reserved.
