p53 Overexpression in lymph node metastases predicts clinical outcome in ductal pancreatic cancer
p53 Overexpression in lymph node metastases predicts clinical outcome in ductal pancreatic cancer
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DOI：
10.1097/00006676-199907000-00004
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发表时间：
1999-07-01
期刊：
影响因子：
2.9
通讯作者：
Fornaciari, G
中科院分区：
文献类型：
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作者：
Campani, D;Boggi, U;Fornaciari, G
在这项研究中，我们评估了133例原发性导管胰腺癌及其区域同步淋巴结转移的p53过表达和增殖活性的预后意义，通过免疫组化，分别使用DO 7和MIB 1单克隆抗体。肿瘤样本和淋巴结是从1976年至1996年手术患者的福尔马林固定、石蜡包埋的档案材料中获得的。患者有良好的临床病史记录，并进行了准确的随访。在133例原发性肿瘤中77例（54%）和50例淋巴结转移患者中22例（44%）观察到p53积聚。p53过表达与原发肿瘤的增殖活性直接相关（p = 0.01）。原发肿瘤和淋巴结转移瘤中p53表达之间存在显著的直接相关性（p = 0.01）; MIB 1的增殖活性也存在同样的相关性（p = 0.002）。患者的总生存率受淋巴结（p = 0.02）和远处（p = 0.0001）转移的影响。淋巴结转移的p53免疫反应性与术后生存期的统计学显著降低相关（p = 0.005）。多变量分析证实了这些结果，只有两个参数保持统计学意义的MI状态（p = 0.0006）和p53过度表达的淋巴结转移（p = 0.01）。
In this study, we evaluated the prognostic significance of both p53 overexpression and proliferating activity in 133 primary ductal pancreatic carcinomas and in their regional synchronous lymph node metastases by immunohistochemistry, by using DO7 and MIB1 monoclonal antibodies, respectively. Tumor samples and lymph nodes were obtained from formalin-fixed, paraffin-embedded archival material of patients operated on between 1976 and 1996. Patients had a well-documented clinical history and were given accurate follow-up. p53 accumulation was observed in 77 (54%) of 133 primary tumors and in 22 (44%) of 50 patients with nodal metastases. The p53 overexpression was directly related to proliferating activity (p = 0.01) in the primary tumors. A significant direct correlation was present between the p53 expression in the primary tumor and in nodal metastases (p = 0.01); the same occurred for proliferating activity by MIB1 (p = 0.002). The patients' overall survival was affected by the presence of nodal (p = 0.02) and distant (p = 0.0001) metastases. The p53 immunoreactivity in nodal metastases was associated with a statistically significant decrease in the postoperative survival period (p = 0.005). Multivariate analysis confirmed these results, and the only two parameters that maintained statistical significance were MI status (p = 0.0006) and p53 overexpression in nodal metastases (p = 0.01).