Phorbol ester-induced expression, phosphorylation, and translocation of protein-tyrosine-phosphatase 1C in HL-60 cells.
Phorbol ester-induced expression, phosphorylation, and translocation of protein-tyrosine-phosphatase 1C in HL-60 cells.
复制标题
佛波酯诱导 HL-60 细胞中蛋白质酪氨酸磷酸酶 1C 的表达、磷酸化和易位。
DOI：
10.1073/pnas.91.11.5007
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发表时间：
1994
影响因子：
11.1
通讯作者：
Fischer,EH
中科院分区：
文献类型：
--
作者：
Zhao,Z;Shen,SH;Fischer,EH
PTP1C 是一种蛋白酪氨酸磷酸酶 (PTP)，含有两个 Src 同源 2 结构域，在 HL-60 人早幼粒细胞白血病细胞中高水平表达。它占核后提取物中总蛋白的 0.15%，相当于在 pH 5.0 下用对硝基苯磷酸盐测量的磷酸酶活性的大约 70%。佛波醇 12-肉豆蔻酸酯 13-乙酸酯 (PMA) 诱导 HL-60 细胞分化为巨噬细胞后，PTP1C 的活性和表达水平增加 2 至 3 倍。此外，PMA 处理后，PTP1C 的丝氨酸残基被磷酸化。虽然该酶完全定位于未处理细胞的胞质部分中，但在分化后发现 30-40% 存在于颗粒部分中。洋地黄皂苷处理前后分化细胞的免疫荧光染色表明结合的酶定位在质膜上。 PTP1C 诱导和易位的时间进程与分化过程相关。原钒酸钠抑制 PMA 诱导的分化，表明 PTP 是该过程所必需的。
PTP1C, a protein-tyrosine-phosphatase (PTP) containing two Src homology 2 domains, is expressed at high levels in HL-60 human promyelocytic leukemia cells. It represents 0.15% of total protein in a postnuclear extract and corresponds to approximately 70% of phosphatase activity measured with p-nitrophenyl phosphate at pH 5.0. Upon differentiation of HL-60 cells to macrophages induced by phorbol 12-myristate 13-acetate (PMA), the activity and expression levels of PTP1C increase 2- to 3-fold. Furthermore, PTP1C is phosphorylated on serine residues upon PMA treatment. While the enzyme localizes entirely in the cytosolic fraction in untreated cells, 30-40% is found in a particulate fraction following differentiation. Immunofluorescent staining of differentiated cells before and after digitonin treatment indicates that the bound enzyme localizes on the plasma membrane. The time courses of induction and translocation of PTP1C correlate with the differentiation process. The PMA-induced differentiation is inhibited by sodium orthovanadate, suggesting the PTPs are required for this process.