Prolactin-Induced Protein facilitates corneal wound healing
Prolactin-Induced Protein facilitates corneal wound healing
复制标题
DOI：
10.1016/j.exer.2022.109300
复制
发表时间：
2022-11-04
影响因子：
3.4
通讯作者：
Karamichos, Dimitrios
中科院分区：
文献类型：
--
作者：
Liang, Wentao;Ma, Jian-Xing;Karamichos, Dimitrios
该研究的目的是研究催乳素诱导蛋白（PIP）在体内和体外角膜伤口愈合中的作用。在 C57BL/6J 小鼠中，使用眼毛刺去除角膜上皮。局部使用磷酸盐缓冲盐水 (PBS) 或 PIP（0.5 和 1.0 μg/mL）或结膜下注射。 PIP 最早可在 24 小时内加速伤口闭合。 PIP 治疗促进角膜伤口愈合以及上皮完整性和厚度。伤口闭合后，PIP 下调整合素 a6、整合素 134、Thrombospondin-1 和 TGF-131 的表达。在体外，使用原代人上皮细胞 (HCEC) 和人角膜成纤维细胞 (HCF) 进行划痕测定，并用不同剂量的 PIP 刺激。 PIP 治疗促进 HCEC 和 HCF 迁移。 PIP 上调 HCEC 中整合素 a6、整合素 134 和 Thrombospondin-1 的表达。在 150 ng/mL PIP 下，HCEC 中 TGF-131 的表达以及 HCF 中平滑肌肌动蛋白 (SMA) 和 III 型胶原 (Col III) 的表达显着下调。 PIP 具有显着的抗纤维化潜力。虽然 PIP 如何影响角膜伤口愈合级联的机制尚不清楚，但我们的发现是新颖的，需要进一步研究以揭示任何治疗潜力。
The purpose of the study was to investigate the role of Prolactin-Induced Protein (PIP) in corneal wound healing, in vivo and in vitro. In C57BL/6J mice, corneal epithelia was removed using an ocular burr. Phosphate buffered saline (PBS) or PIP (0.5 and 1.0 mu g/mL) was applied topically or subconjunctivally injected. PIP accelerated wound closure as early as 24 h. PIP treatment promoted corneal wound healing and epithelial integrity and thickness. Integrin a6, integrin 134, Thrombospondin-1, and TGF-131 expressions were all downregulated by PIP after wound closure. In vitro, scratch assays were performed using primary human epithelial cells (HCECs) and human corneal fibroblasts (HCFs), stimulated with PIP at various dosages. PIP treatment promoted both HCECs and HCFs migration. PIP upregulated expression of integrin a6, integrin 134, and Thrombospondin-1 in HCECs. Expression of TGF-131 in HCECs and expression of smooth muscle actin (SMA) and Type III Collagen (Col III) in HCFs were significantly downregulated at 150 ng/mL PIP. PIP exhibits noteworthy anti-fibrotic potentiality. While the mechanism of how PIP is impactful on the corneal wound healing cascade is unknown, our findings are novel and further studies are warranted in order to unravel any therapeutic potential.