Lipid mobility and molecular binding in fluid lipid membranes.
Lipid mobility and molecular binding in fluid lipid membranes.
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流体脂质膜中的脂质流动性和分子结合。
DOI：
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发表时间：
2005
影响因子：
15
通讯作者：
J. Groves
中科院分区：
文献类型：
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作者：
Victoria Yamazaki;O. Sirenko;R. J. Schafer;J. Groves
研究发现，在膜表面受体配体结合时，即使脂质不直接参与结合事件，掺入支撑膜的稀浓度荧光标记脂质的横向迁移率也会发生变化。使用膜微阵列进行实验，说明使用脂质迁移率测量作为检测膜表面结合的有效无标记策略。
The lateral mobility of dilute concentrations of fluorescently labeled lipids doped into supported membranes is found to change upon receptor ligand binding at the membrane surface, even when the lipid is not directly involved in the binding event. Experiments using membrane microarrays are performed that illustrate the use of lipid mobility measurements as an effectively label-free strategy of detecting binding on membrane surfaces.