Interaction of human spermatozoa with the human zona pellucida and zona-free hamster oocyte following capacitation by exposure to human cervical mucus
Interaction of human spermatozoa with the human zona pellucida and zona-free hamster oocyte following capacitation by exposure to human cervical mucus
复制标题
暴露于人宫颈粘液获能后人精子与人透明带和无带仓鼠卵母细胞的相互作用
DOI：
10.1002/mrd.1120120106
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
F. Hanson
中科院分区：
文献类型：
--
作者：
J. Gould;J. Overstreet;F. Hanson
将人精子通过人宫颈黏液使其失能后，检测精子与人透明带和无带仓鼠卵母细胞相互作用的功能能力。将结果与从同一精液样本中提取的精子通过标准实验室方法（通过稀释和离心对精液进行顺序洗涤）获得的结果进行比较。洗涤获能精子悬浮液比黏液获能精子更成功地附着在无带的仓鼠卵母细胞上并与卵膜融合。然而，黏液活化精子穿透人体透明带的能力与水洗活化精子相同。这些实验证明了一种可能在体内和体外比较研究人类精子获能的方法。
The functional capacity for sperm interaction with the human zona pellucida and zona-free hamster oocyte was tested after human spermatozoa were capacitated by passage through a column of human cervical mucus. The results were compared with those obtained when spermatozoa from an aliquot of the same semen sample were capacitated by the standard laboratory methods involving sequential washing by dilution and centrifugation of the semen. Washed-capacitated sperm suspensions were more successful than mucus-capacitated sperm in attaching to the zona-free hamster oocyte and in fusing with its oolemma. However, the ability of mucus-capacitated sperm to penetrate the human zona pellucida was equal to washed capacitated sperm. These experiments demonstrate an approach that may be useful in comparative studies of human sperm capacitation in vivo and in vitro.
人类女性生殖道中的精子获能。
影响因子：
6.7
作者：
Lambert,H;Overstreet,JW;Morales,P;Hanson,FW;Yanagimachi,R
通讯作者：
Yanagimachi,R