Cellular retinoic acid-binding proteins in human epidermis and sebaceous follicles.
Cellular retinoic acid-binding proteins in human epidermis and sebaceous follicles.
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人表皮和皮脂囊中的细胞视黄酸结合蛋白。
DOI：
10.1111/1523-1747.ep12259164
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
M. Sakamoto
中科院分区：
文献类型：
--
作者：
S. M. Puhvel;M. Sakamoto
有证据表明，类维甲酸对敏感靶组织的一些作用是由特定的细胞结合蛋白介导的。本研究证实了细胞维甲酸结合蛋白（cRABP）在人体皮肤中的存在。采用氚化维甲酸（RA）和氚化13-顺式维甲酸（13-顺式RA）作为放射性标记配体，独立测定了cRABP在表皮和皮脂腺滤泡细胞质中的近似浓度和亲和力。Scatchard分析表明，以每mg细胞质蛋白的结合量为基础，表皮和皮脂腺卵泡细胞质对RA和13-顺式RA的结合能力相似。然而，13-顺式RA与皮脂腺卵泡cRABP结合的解离常数是RA Kd的9倍以上（1096 nM比117 nM）。表皮cRABP结合13-cis-RA的Kd是结合RA的31倍（3582 nM比114 nM）。因此，表皮和皮脂腺毛囊对13-顺式RA的亲和力明显低于对RA的亲和力。似乎人皮脂腺对13-顺式ra反应的特异性取决于这些结构中cRABP的浓度或亲和力以外的其他因素。
Evidence suggests that some of the effects of retinoids on sensitive target tissues are mediated by specific cellular binding proteins. This study demonstrated the presence of cellular retinoic acid-binding proteins (cRABP) in human skin. The approximate concentration and affinity of cRABP was determined independently in epidermal and sebaceous follicle cytosol, using both tritiated retinoic acid (RA) and tritiated 13-cis-retinoic acid (13-cis-RA) as radiolabeled ligands. Scatchard analysis suggested that on the basis of binding per mg of cytosol protein, the binding capacities by epidermal and sebaceous follicle cytosol were similar for RA and 13-cis-RA. However, the dissociation constants of binding of 13-cis-RA by sebaceous follicle cRABP was more than 9-fold the Kd for RA (1096 nM compared to 117 nM). The Kd for binding 13-cis-RA by epidermal cRABP was 31-fold the Kd for binding RA (3582 nM compared to 114 nM). Thus the affinity for 13-cis- RA by epidermal and sebaceous follicle cRABP is significantly less than for RA. It appears that the specificity of response of human sebaceous glands to 13-cis-RA is dependent on factors other than the concentration or affinity of cRABP in these structures.