S100 IS PREFERENTIALLY DISTRIBUTED IN MYELIN-FORMING SCHWANN-CELLS
S100 IS PREFERENTIALLY DISTRIBUTED IN MYELIN-FORMING SCHWANN-CELLS
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DOI：
10.1007/bf01188409
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发表时间：
1990-06-01
期刊：
影响因子：
--
通讯作者：
FINK, DJ
中科院分区：
文献类型：
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作者：
MATA, M;ALESSI, D;FINK, DJ
为了阐明S100蛋白在体内的作用，我们使用了植入后电子显微镜免疫细胞化学与市售的抗体S100，和第二抗体结合胶体金，以确定在坐骨神经和颈交感神经干的大鼠的S100蛋白的分布。我们发现，S100免疫反应在雪旺细胞的差异分布。有髓纤维中S100免疫反应性的量似乎与由雪旺细胞形成的髓鞘的厚度直接相关。无髓纤维与小的有髓纤维在S100免疫反应性上相同。在Schwann细胞内，免疫反应性见于细胞核和核周细胞质，以及Schmidt-Lanterman裂隙和Ranvier结旁环，但不在髓鞘本身。使用这些抗体，我们没有看到可重复的或令人信服的轴突内免疫反应在正常神经。这些结果表明，在雪旺细胞中，S100的表达可能与轴突直径和髓鞘形成程度有关。S100在发育或损伤后的表达的进一步研究可能会阐明S100在雪旺细胞生物学中的作用。
In order to elucidate the role of S100 protein in vivo we used postembedding electron microscopical immunocytochemistry with commercially available antibodies to S100, and secondary antibodies conjugated to colloidal gold to determine the distribution of S100 protein in the sciatic nerve and the cervical sympathetic trunk of the rat. We found that S100 immunoreactivity in Schwann cells was differentially distributed. The amount of S100 immunoreactivity in myelinated fibres appeared to correlate directly with the thickness of the myelin sheath formed by the Schwann cell. Unmyelinated fibres were identical to the small myelinated fibres in S100 immunoreactivity. Within the Schwann cells, the immunoreactivity was seen in the nucleus and in the perikaryal cytoplasm, as well as in the Schmidt-Lanterman clefts and in the paranodal loops at the node of Ranvier, but not in the myelin itself. Using these antibodies we did not see reproducible or convincing intra-axonal immunoreactivity in normal nerve. These results suggest that in Schwann cells S100 expression may be related to axon diameter and degree of myelination. Future studies of S100 expression in development or in response to injury may clarify the role of S100 in Schwann cell biology.