Non-destructive method for determining temporal and spatial changes of the soil solution chemistry in the rhizosphere
Non-destructive method for determining temporal and spatial changes of the soil solution chemistry in the rhizosphere
复制标题
确定根际土壤溶液化学时空变化的无损方法
DOI：
10.1080/00380768.2000.10409136
复制
发表时间：
2000
影响因子：
2
通讯作者：
T. Kosaki
中科院分区：
文献类型：
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作者：
N. Moritsuka;J. Yanai;T. Kosaki
摘要 提出了一种以高空间分辨率对根际土壤溶液进行无损采样的方法。能够分离根际土壤的根箱与带有环形中空纤维的土壤溶液采样器（LHF采样器）相结合。在根箱中，LHF 采样器安装在距离根表面 0-2 毫米和 8-10 毫米的位置。盆栽试验进行了 17 天，有两种处理，即玉米 (Zea mays L.) 和无植物。在实验过程中，在第 5、9、13 和 17 天收集土壤溶液，并分析离子浓度。实验结束后，距根面每隔2 mm采集土壤样品，直至10 mm，并分析可溶性离子浓度，以评估土壤溶液取样造成的干扰程度。本实验中应用的方法能够以高空间分辨率、最小干扰对根际土壤溶液进行采样。随着植物的生长，距根面0~2 mm处采集的土壤溶液中除SO4 2−外的主要离子浓度显着低于8~10 mm处的浓度。该过程导致离子组成发生改变，即仅在距根部表面几毫米的范围内以 SO4 2− 而不是 NO3 − 为主。这些定量结果表明，植物根部吸收根际土壤溶液，其中NO3 - 的水平相对低于外部溶液的水平，而其他阴离子如SO4 2− 在调节阳离子浓度及其对植物的供应方面发挥着重要作用。
Abstract A non-destructive method for sampling the rhizosphere soil solution at a high spatial resolution is presented. A rhizobox, which enables to separate soils in the rhizosphere, was combined with soil solution samplers with a looped hollow fiber (LHF-sampler). In a rhizobox, LHF-samplers were installed at a distance of 0–2 mm and 8–10 mm from the root surface. A pot experiment was carried out for 17 d with two treatments, i.e. maize (Zea mays L.) and no plants. During the experiment, soil solutions were collected on day 5,9,13, and 17, and analyzed for the ionic concentrations. After the experiment, the soil samples were also collected at a distance of every 2 mm from the root surface up to 10 mm and analyzed for soluble ion concentrations in order to evaluate the degree of disturbance due to soil solution sampling. The method applied in this experiment enabled to sample the soil solution from the rhizosphere at a high spatial resolution with minimal disturbance. With plant growth, the concentrations of major ions except for SO4 2− in the soil solution collected at a distance of 0–2 mm from the root surface became significantly lower than those at 8–10 mm. This procedure led to the alteration of the ionic composition, i.e. the predominance of SO4 2− instead of NO3 −, only within a few millimeters from the root surface. These quantitative results suggested that plant roots absorb the soil solution in the rhizosphere where the level of NO3 − is relatively lower than that of the outer solution and that other anions such as SO4 2− play an important role in regulating the concentrations of cations and their supply to plants.
KYUMA，Kazutake：土壤科学和植物营养。
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