Proteomic analysis of protein expression profiles during hyperthermia-induced apoptosis in Tca8113 cells.
Proteomic analysis of protein expression profiles during hyperthermia-induced apoptosis in Tca8113 cells.
复制标题
Tca8113细胞热诱导凋亡过程中蛋白质表达谱的蛋白质组学分析
DOI：
10.3892/ol.2013.1354
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发表时间：
2013-07
期刊：
影响因子：
2.9
通讯作者：
He Y
中科院分区：
文献类型：
--
作者：
Jiang W;Bian L;Wang N;He Y
本研究的目的是探讨热疗诱导癌细胞凋亡过程中的蛋白质表达谱。使用源自人舌鳞状细胞癌的Tca8113细胞系建立热疗诱导的细胞凋亡模型，并在热疗诱导后2、6、8、12和24小时进行荧光差异显示二维（2D）凝胶电泳。通过质谱分析鉴定蛋白质。通过蛋白质印迹分析检测蛋白质的表达变化。与对照组相比，热疗处理的细胞中总共检测到 107 个蛋白质，这些蛋白质表现出不同的表达水平，其中 57 个蛋白质被鉴定。通过蛋白质印迹分析进一步验证了代表性蛋白质的表达变化。这57个蛋白质根据以下功能组进行鉴定：能量代谢相关酶、细胞骨架相关蛋白、分子伴侣、转录因子、蛋白质合成相关蛋白以及细胞分裂和增殖相关蛋白。这些组包括 44 个上调蛋白和 13 个下调蛋白。 44个上调蛋白中，27个持续上调，8个早期时间点上调，9个中后期上调。 13个下调的蛋白中，5个持续下调，6个早期时间点下调，2个中后期下调。这些结果表明，热疗诱导的Tca8113细胞凋亡受多种因素控制，其中包括时间和调节蛋白。
The aim of the present study was to explore protein expression profiles during cancer cell apoptosis induced by hyperthermia. A hyperthermia-induced apoptosis model was established using a Tca8113 cell line derived from a human tongue squamous cell carcinoma, which underwent fluorescent differential display two-dimensional (2D) gel electrophoresis at 2, 6, 8, 12 and 24 h following the induction of hyperthermia. Proteins were identified by mass spectrometry analysis. Expression changes in the proteins were detected by western blot analysis. A total of 107 proteins were detected that exhibited different expression levels in the hyperthermia-treated cells compared with the controls, and 57 of these proteins were identified. Expression changes in the representative proteins were further verified by western blot analysis. These 57 proteins were identified according to the following functional groups: energy metabolism-related enzymes, cytoskeleton-related proteins, chaperones, transcription factors, protein synthesis-related proteins and cell division- and proliferation-related proteins. These groups included 44 upregulated and 13 downregulated proteins. Among the 44 upregulated proteins, 27 were upregulated continuously, eight were upregulated at an early time-point and nine were upregulated at a middle to late time-point. Among the 13 downregulated proteins, five were downregulated continuously, six were downregulated at an early time-point and two were downregulated at a middle to late time-point. These results indicate that hyperthermia-induced Tca8113 cell apoptosis is controlled by multiple factors, which include time and regulatory proteins.
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影响因子：
4
作者：
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通讯作者：
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