Embryonic Stem Cells Do Not Stiffen on Rigid Substrates
Embryonic Stem Cells Do Not Stiffen on Rigid Substrates
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DOI：
10.1016/j.bpj.2010.04.057
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发表时间：
2010-07-21
影响因子：
3.4
通讯作者：
Wang, Ning
中科院分区：
文献类型：
--
作者：
Poh, Yeh-Chuin;Chowdhury, Farhan;Wang, Ning
先前已经确定，包括间充质干细胞在内的活细胞响应于基底刚度的升高而伸展。这种硬化在很大程度上归因于细胞基底处的牵引力的升高，该牵引力与细胞在更刚性的基底上扩展的增加相关。令人惊讶的是，我们在这里发现，小鼠胚胎干细胞（ESCs）不会随着基质硬度的增加而增加。如最近所示，这些细胞也不会增加在更刚性的基底上的铺展。然而，这些ESC确实随着基底刚度的增加而增加它们的基底牵引力。我们的结论是，这些胚胎干细胞表现出不同的间充质干细胞和终末分化的细胞的力学行为，并解耦其顶端细胞刚度从其基底牵引应力在基板刚度响应。
It has been previously established that living cells, including mesenchymal stem cells, stiffen in response to elevation of substrate stiffness. This stiffening is largely attributed to the elevation of the tractions at the cell base that is associated with increases in cell spreading on more-rigid substrates. We show here, surprisingly, that mouse embryonic stem cells (ESCs) do not stiffen when substrate stiffness increases. As shown recently, these cells do not increase spreading on more-rigid substrates either. However, these ESCs do increase their basal tractions as substrate stiffness increases. We conclude that these ESCs exhibit mechanical behaviors distinct from those of mesenchymal stem cells and of terminally differentiated cells, and decouple its apical cell stiffness from its basal tractional stresses during the substrate rigidity response.