Mechanism of inactivation of chymotrypsin by 5-butyl-3H-1,3-oxazine-2,6-dione.
Mechanism of inactivation of chymotrypsin by 5-butyl-3H-1,3-oxazine-2,6-dione.
复制标题
5-丁基-3H-1,3-恶嗪-2,6-二酮灭活胰凝乳蛋白酶的机制。
DOI：
10.1021/bi00306a008
复制
发表时间：
1984
期刊：
影响因子：
2.9
通讯作者：
Abeles,RH
中科院分区：
文献类型：
--
作者：
Weidmann,B;Abeles,RH
方法分析方法。所有的分光光度测定均用恒温于25 ℃的Perkin-Elmer Lamda 3紫外-可见分光光度计进行。对于放射性测定，将样品溶解于10 mL闪烁液（ACS，阿默舍姆）中，并在Beckman LS-100 C液体闪烁系统上计数。通过加入14 C标记的标准品（New
MethodsAnalytical Procedures. All spectrophotometric determinations were made with a Perkin-Elmer Lamda 3 UV-vis spectrometer thermostated at 25 C. For determination of radioactivity, samples were dissolved in 10 mL of scintillation fluid (ACS, Amersham) and counted on a Beckman LS-100 C liquid scintillation system. The countingefficiency was determined by addition of a 14C-labeled standard (New