Fluorescent Analysis of the Cell-Selective Alzheimer's Disease Aβ Peptide Surface Membrane Binding: Influence of Membrane Components
Fluorescent Analysis of the Cell-Selective Alzheimer's Disease Aβ Peptide Surface Membrane Binding: Influence of Membrane Components
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细胞选择性阿尔茨海默病 Aβ 肽表面膜结合的荧光分析：膜成分的影响
DOI：
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发表时间：
2011
影响因子：
--
通讯作者：
N. Arispe
中科院分区：
文献类型：
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作者：
O. Šimáková;N. Arispe
We performed a fluorescent analysis of the binding of Aβ to the surface membrane of different types of cells lines such as PC12, GT1-7, and ex vivo neurons. Analyses were performed on sorted cells with membrane bound Aβ Competitive binding between Aβ phosphatidyl serine- (PtdSer-) specific binder annexin V and an anti-PtdSer antibody provided compelling data confirming the involvement of PtdSer as one of the surface membrane signal molecules for Aβ. We found that populations of cells that exhibited high surface membrane binding affinity for Aβ also show higher membrane cholesterol levels compared to cells that did not bind Aβ. This direct relationship was upheld in cholesterol-enriched or cholesterol-depleted cell membranes. We conclude that the initial process for the cell-selective binding by Aβ, to later conversion of elemental Aβ units into larger structures such as fibrils or to the potentially toxic ion channel aggregates, is highly influenced by the membrane content of PtdSer and cholesterol in the cell surface membrane.
磷脂酰丝氨酸和磷脂酰乙醇胺的荧光类似物在培养细胞质膜上的跨双层运动。
DOI：
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发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Martin,OC;Pagano,RE
通讯作者：
Pagano,RE