Fetal liver myelopoiesis occurs through distinct, prospectively isolatable progenitor subsets.
Fetal liver myelopoiesis occurs through distinct, prospectively isolatable progenitor subsets.
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DOI：
10.1182/blood.v98.3.627
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发表时间：
2001-08
期刊：
影响因子：
20.3
通讯作者：
D. Traver;T. Miyamoto;Julie Anja Engelhard Christensen;Junko Iwasaki-Arai;Koichi Akashi;I. Weissman
中科院分区：
文献类型：
--
作者：
D. Traver;T. Miyamoto;Julie Anja Engelhard Christensen;Junko Iwasaki-Arai;Koichi Akashi;I. Weissman
发育中的小鼠胚胎的造血命运图仍然不精确。成人型造血首先出现在胎儿肝脏，然后发展到脾脏和骨髓。最近已经鉴定了成年小鼠骨髓中的克隆性共同淋巴样祖细胞和克隆性共同髓样祖细胞（CMPs），其分别产生所有淋巴样和髓样谱系。在这里，它表明，骨髓细胞生成在胎儿肝脏类似地进行通过CMP等价物。胎肝CMP产生巨核细胞-红细胞限制性祖细胞（MEP）和粒细胞-单核细胞限制性祖细胞（GMP），其也可以通过细胞表面表型前瞻性地分离。MEP和GMP在克隆形成集落测定和移植实验中产生相互排斥的细胞类型，表明在正常条件下在每个祖细胞亚群内观察到的谱系限制是绝对的。纯化的祖细胞群体用于分析各种造血相关基因的表达谱。表达模式与成年对应人群的表达模式密切匹配。这些结果表明，成人造血系统的层次结构确定在发展的早期的永久性免疫系统，并表明，骨髓红细胞谱系内的细胞命运决定的分子机制是保守的，从胚胎到成人。（血。2001;98：627-635）
Hematopoietic fate maps in the developing mouse embryo remain imprecise. Definitive, adult-type hematopoiesis first appears in the fetal liver, then progresses to the spleen and bone marrow. Clonogenic common lymphoid progenitors and clonogenic common myeloid progenitors (CMPs) in adult mouse bone marrow that give rise to all lymphoid and myeloid lineages, respectively, have recently been identified. Here it is shown that myelopoiesis in the fetal liver similarly proceeds through a CMP equivalent. Fetal liver CMPs give rise to megakaryocyte-erythrocyte-restricted progenitors (MEPs) and granulocyte-monocyte-restricted progenitors (GMPs) that can also be prospectively isolated by cell surface phenotype. MEPs and GMPs generate mutually exclusive cell types in clonogenic colony assays and in transplantation experiments, suggesting that the lineage restriction observed within each progenitor subset is absolute under normal conditions. Purified progenitor populations were used to analyze expression profiles of various hematopoiesis-related genes. Expression patterns closely matched those of the adult counterpart populations. These results suggest that adult hematopoietic hierarchies are determined early in the development of the definitive immune system and suggest that the molecular mechanisms underlying cell fate decisions within the myeloerythroid lineages are conserved from embryo to adult. (Blood. 2001;98:627-635)