Role of molecular size in ratchet fractionation
Role of molecular size in ratchet fractionation
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DOI：
10.1103/physrevlett.89.178301
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发表时间：
2002-10-21
影响因子：
8.6
通讯作者：
Craighead, H
中科院分区：
文献类型：
--
作者：
Huang, LR;Silberzan, P;Craighead, H
我们展示了在微流体不对称连续流扩散阵列中有限颗粒尺寸的重要性，特别是相对于屏障间隙的颗粒尺寸的临界性质。我们发现，粒子远小于势垒间隙遵循个别的场线通过狭窄的差距，并不好分馏。相反，与差距尺寸相当的粒子失去了它们的入射场线的记忆，并且可以以高分辨率被分离。我们证明了这种效果，使用一种新的技术方法，以创建非常直和狭窄的注射带在这样的阵列，并完全解决带的长度为48 500和167 000碱基对的DNA。
We show the importance of finite particle size in microfluidic asymmetric continuous-flow diffusion arrays, specifically the critical nature of the particle size with respect to the barrier gaps. We show that particles much smaller than the barrier gap follow individual field lines through narrow gaps and are poorly fractionated. In contrast, particles comparable to the gap size lose memory of their incoming field line and can be fractionated with high resolution. We demonstrate this effect using a new technological approach to create very straight and narrow injection bands in such arrays, and completely resolve bands of DNA of lengths 48 500 and 167 000 base pairs.