Comparison of structural characterization and antioxidant activity of polysaccharides from jujube (Ziziphus jujuba Mill.) fruit
Comparison of structural characterization and antioxidant activity of polysaccharides from jujube (Ziziphus jujuba Mill.) fruit
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DOI：
10.1016/j.ijbiomac.2020.02.018
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发表时间：
2020-04-15
影响因子：
8.2
通讯作者：
Liu, Yanqi
中科院分区：
文献类型：
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作者：
Ji, Xiaolong;Hou, Chunyan;Liu, Yanqi
以枣（Ziziphus ereba Mill.）并进一步比较它们的结构特征和抗氧化活性。首先，从红枣中分离纯化了三种多糖，命名为PZMP 1。通过DEAE-Sepharose Fast Flow和Sephaayl 5-300柱成功地制备了PZMP 2和PZMP 3。通过化学测试、高效凝胶渗透色谱（HPGPC）、气相色谱（GC）、傅立叶变换红外光谱（FT-IR）、甲基化分析、核磁共振谱、X射线衍射（XRD）、扫描电子显微镜（SEM）和原子力显微镜（AIM）等手段对它们的特性进行了研究和比较。并对它们的抗氧化活性进行了比较。结果表明，DEAE-Sepharose Fast Flow和Sephacryl 5-300柱吸附量不同的3种大枣多糖具有不同的结构特征和抗氧化活性，且半乳糖醛酸含量较高的大枣多糖具有较好的抗氧化活性，为大枣多糖的功能性和药用开发提供了一定的方向。(C)2020由Elsevier B. V.出版
The aim of this paper was to prepare purified fractions of polysaccharides from jujube (Ziziphus jujuba Mill.) fruit and further compare their structural characteristics and antioxidant activities. Firstly, three purified jujube polysaccharides, named PZMP1. PZMP2 and PZMP3, were successfully prepared by DEAE-Sepharose Fast Flow and Sephaayl 5-300 columns. Then, their characteristics were studied and then compared by using chemical testing, high-performance gel permeation chromatography (HPGPC), gas chromatography (GC), Fourier transform infrared spectroscopy (FT-IR), methylation analysis, NMR spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM) and atomic force microscopy (AIM). Thereafter, their antioxidant activities were also contrasted. The data achieved demonstrated that the three jujube polysaccharides derived from different adsorption capacity of DEAE-Sepharose Fast Flow and Sephacryl 5-300 columns possessed different structural characteristics and antioxidant activity, and with higher galacturonic acid content could exhibit better antioxidant bioactivities, which could provide certain directions for the functional and medicinal purposes development of jujube polysaccharides. (C) 2020 Published by Elsevier B.V.