Immunologic synergism with IL-2 and effects of cCHMIs on mRNA expression of IL-2 and IFN-γ in chicken peripheral T lymphocyte
Immunologic synergism with IL-2 and effects of cCHMIs on mRNA expression of IL-2 and IFN-γ in chicken peripheral T lymphocyte
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DOI：
10.1016/j.vaccine.2006.07.005
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发表时间：
2006-11-30
期刊：
影响因子：
5.5
通讯作者：
Liu, Jiaguo
中科院分区：
文献类型：
--
作者：
Wang, Deyun;Li, Xiangrui;Liu, Jiaguo
分别用淫羊藿多糖+蜂胶黄酮(cCHMIs 1)和黄芪多糖+人参皂苷(cCHMIs 2)制备两种复方中药成分(cCHMIs)。动物免疫实验中，将新城疫疫苗分别与两种cCHMIs和IL-2混合，接种15日龄鸡实验组。接种后第7、14、21、28天，采用微量法检测血清抗体滴度的动态变化。在基因表达实验中，将每种cCHMIs以三种浓度添加到培养的鸡外周T淋巴细胞中。培养7 h后，半定量RT-PCR法测定细胞中IL-2 mRNA的表达，培养24 h后，IFN-γ mRNA的表达。结果显示，两种cCHMI无论是单独注射还是与IL-2联合注射，均能显着提高抗体滴度，而两种cCHMI与IL-2的免疫协同作用不明显。 3个剂量的cCHMs 1和高剂量的cCHMIs 2可以显着促进鸡T淋巴细胞IL-2 mRNA的表达，3个剂量的2个cCHMIs可以显着促进IFN-γ mRNA的表达，这可以解释为什么cCHMIs与IL-2的免疫协同作用不明显。 (c) 2006 Elsevier Ltd. 保留所有权利。
Two compound Chinese herbal medicinal ingredients (cCHMIs) were prepared, respectively, with epimedium polysaccharide plus propolis flavone (cCHMIs 1) and astragalus polysaccharide plus ginsenoside (cCHMIs 2). In animal immune experiment, Newcastle disease vaccine was mixed, respectively, with two cCHMIs and IL-2 to vaccinate 15-day-old chicken in experimental groups. On days 7, 14, 21 and 28 after vaccination, the dynamic changes of serum antibody titers were tested by micro-method. In gene expression experiment, each cCHMIs, at three concentrations, was added into cultured chicken peripheral T lymphocyte. After cultivation of 7 h, the expression of IL-2 mRNA in the cell, 24 h, IFN-gamma mRNA, were determined by semi-quantitative RT-PCR assay. The results showed that two cCHMIs, whether single or cooperative injection with IL-2, could significantly raise the antibody titers, while immunologic synergisms of two cCHMIs with IL-2 were unconspicuous. cCHMs 1 at three doses and cCHMIs 2 at high dose could remarkably promote the expression of IL-2 mRNA in chicken T lymphocyte, two cCHMIs at three doses, the expression of IFN-gamma mRNA, which could explain why the immunologic synergism of cCHMIs with IL-2 was not obvious. (c) 2006 Elsevier Ltd. All rights reserved.