THE BRAIN AND THE IMMUNE-SYSTEM - AN INTACT IMMUNE-SYSTEM IS ESSENTIAL FOR THE MANIFESTATION OF WITHDRAWAL IN OPIATE ADDICTED RATS
THE BRAIN AND THE IMMUNE-SYSTEM - AN INTACT IMMUNE-SYSTEM IS ESSENTIAL FOR THE MANIFESTATION OF WITHDRAWAL IN OPIATE ADDICTED RATS
复制标题
DOI：
10.1016/0306-4522(90)90293-d
复制
发表时间：
1990-01-01
期刊：
影响因子：
3.3
通讯作者：
DAFNY, N
中科院分区：
文献类型：
--
作者：
DOUGHERTY, PM;PELLIS, NR;DAFNY, N
γ射线照射（500拉德）通常用于抑制小鼠、大鼠和人的免疫系统。最近，研究表明，在慢性吗啡治疗前进行照射，可显著降低吗啡依赖动物纳洛酮催促戒断的严重程度。在本研究中，过继转移2-6倍。108脾细胞的辐射大鼠慢性吗啡治疗前恢复了所有戒断症状的严重程度，由纳洛酮沉淀。相比之下，过继转移分级脾细胞亚群仅部分恢复戒断严重程度;和辐射脾细胞，红细胞或正常脾细胞的稀释数量的转移没有任何观察到的恢复效果。这些发现表明，特定的细胞活动或来自淋巴细胞的因子是阿片类药物戒断的表达所必需的。
Gamma irradiation (500 rad) is often used to suppress the immune system in mice, rats and man. Recently, it was shown that irradiation prior to chronic morphine treatment, dramatically reduces the severity of naloxone-precipitated withdrawal in morphine-dependent animals. In the present study adoptive transfer of 2-6 .times. 108 splenocytes to irradiated rats prior to chronic morphine treatment restored the severity of all withdrawal signs precipitated by naloxone. In contrast, adoptive transfer of fractionated splenocyte subpopulations only partially restored withdrawal severity; and transfer of irradiated splenocytes, red blood cells or diluted numbers of normal splenocytes did not have any observed restorative effect. These findings suggest that specific cellular activities or factors derived from lymphoid cells are required for the expression of opiate withdrawal.