Overexpression of a novel salt stress-induced glycine-rich protein gene from alfalfa causes salt and ABA sensitivity in Arabidopsis
Overexpression of a novel salt stress-induced glycine-rich protein gene from alfalfa causes salt and ABA sensitivity in Arabidopsis
复制标题
DOI：
10.1007/s00299-013-1443-0
复制
发表时间：
2013-08-01
影响因子：
6.2
通讯作者：
Zhang, Ze
中科院分区：
文献类型：
--
作者：
Long, Ruicai;Yang, Qingchuan;Zhang, Ze
我们从苜蓿中克隆了一个新的盐胁迫诱导的富含甘氨酸蛋白基因（MsGRP）。盐胁迫和阿坝处理后，转基因拟南芥的种子萌发和幼苗生长受到抑制。由于土壤盐分是最重要的非生物胁迫之一，因此需要耐盐性来克服盐分诱导的作物生产力下降。许多富含甘氨酸的蛋白质（GRP）参与了植物对环境胁迫的反应，但一些GRP在胁迫反应中的功能和重要性仍不清楚。在这里，我们报告了一个新的盐胁迫诱导的GRP基因（MsGRP），我们从苜蓿中分离。与一些富含甘氨酸的RNA结合蛋白相比，MsGRP不含RNA识别基序，亚细胞定位结果显示其定位于细胞膜或细胞壁。MsGRP mRNA诱导盐，脱落酸（阿坝），和干旱胁迫在苜蓿幼苗，其过表达驱动的组成型花椰菜花叶病毒-35S启动子在拟南芥植物中赋予盐和阿坝的敏感性相比，野生型植物。MsGRP抑制盐和阿坝处理后转基因拟南芥种子的萌发和幼苗的生长，表明MsGRP可能通过ABA依赖的调控途径影响种子的萌发和幼苗的生长。这些结果间接证明了MsGRP在某些非生物胁迫条件下对苜蓿种子萌发和幼苗生长具有重要作用。
We cloned a novel salt stress-induced glycine-rich protein gene ( MsGRP ) from alfalfa. Its overexpression retards seed germination and seedling growth of transgenic Arabidopsis after salt and ABA treatments.Since soil salinity is one of the most significant abiotic stresses, salt tolerance is required to overcome salinity-induced reductions in crop productivity. Many glycine-rich proteins (GRPs) have been implicated in plant responses to environmental stresses, but the function and importance of some GRPs in stress responses remain largely unknown. Here, we report on a novel salt stress-induced GRP gene (MsGRP) that we isolated from alfalfa. Compared with some glycine-rich RNA-binding proteins, MsGRP contains no RNA recognition motifs and localizes in the cell membrane or cell wall according to the subcellular localization result. MsGRP mRNA is induced by salt, abscisic acid (ABA), and drought stresses in alfalfa seedlings, and its overexpression driven by a constitutive cauliflower mosaic virus-35S promoter in Arabidopsis plants confers salinity and ABA sensitivity compared with WT plants. MsGRP retards seed germination and seedling growth of transgenic Arabidopsis plants after salt and ABA treatments, which implies that MsGRP may affect germination and growth through an ABA-dependent regulation pathway. These results provide indirect evidence that MsGRP plays important roles in seed germination and seedling growth of alfalfa under some abiotic stress conditions.