Prospective, preclinical comparison of the performance between radiofrequency microneedling and microneedling alone in reversing photoaged skin
Prospective, preclinical comparison of the performance between radiofrequency microneedling and microneedling alone in reversing photoaged skin
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DOI：
10.1111/jocd.13116
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发表时间：
2019-09-06
影响因子：
2.3
通讯作者：
Kim, Beom Joon
中科院分区：
文献类型：
--
作者：
Hong, Ji Yeon;Kwon, Tae-Rin;Kim, Beom Joon
背景对于具有最小侵入性和患者停机时间的真皮再生有巨大的需求。目的在这项研究中，我们评估了非分割单极射频治疗光老化皮肤纹理和皱纹的性能。方法将32只6周龄雌性无毛小鼠随机分为4组，每组8只：（a）健康对照组，（B）UVB暴露组，（c）UVB +微针照射组，（d）UVB +微针RF照射组。应用每种治疗方式后，皮肤表面进行了全面调查和组织学评价老年皮肤变化。免疫组化用胶原I型和III型的一抗进行测试。结果UVB照射后，皮肤Ra值明显升高，出现干燥性鳞屑皱纹。在RF治疗的小鼠中，皱纹的深度和数量显示出逐渐改善。RF治疗组的平均Ra值显著降低。RF治疗组表皮厚度减少，真皮炎性细胞浸润受到抑制，胶原纤维密度和弹性纤维数量增加。结论针刺射频治疗可改善光老化小鼠皮肤纹理和皱纹。据我们所知，我们的研究结果提供了第一个证据表明，非分割单极微针射频装置可能有助于治疗紫外线损伤的皮肤。
Background There is a tremendous demand for dermal rejuvenation with minimal invasiveness and patient downtime. Aims In this study, we evaluated the performance of nonfractional monopolar radiofrequency for the improvement of photoaged skin texture and wrinkles. Methods In total, 32 6-week-old female hairless mice were randomized into four groups of eight mice each: (a) healthy control, (b) UVB-exposed, (c) UVB + microneedling, and (d) UVB + microneedle RF. After applying each treatment modality, skin surface was globally investigated and histologically evaluated senile skin change. Immunohistochemistry was tested with the primary antibody to collagen type I and III. Results After UVB exposure, the Ra value was significantly increased, leading to clinical development of wrinkles with xerotic scales. Depth and number of wrinkles showed gradual improvement in RF-treated mice. The mean Ra value of the RF-treated group decreased significantly. The RF-treated group showed decreased epidermal thickness, suppression of dermal inflammatory cell infiltration, and increased density of collagen fibers and amount of elastic fibers. Conclusions Microneedle RF treatment alleviates photoaged skin texture and wrinkles in this mouse model. To the best of our knowledge, our results provide the first evidence that a nonfractional monopolar microneedle radiofrequency device may contribute to the treatment of UV-damaged skin.