Mitogenic effects of bacterial cell walls and their components on murine splenocytes.
Mitogenic effects of bacterial cell walls and their components on murine splenocytes.
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细菌细胞壁及其成分对小鼠脾细胞的有丝分裂作用。
DOI：
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
I. Yano
中科院分区：
文献类型：
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作者：
H. Takada;S. Nagao;S. Kotani;S. Kawata;K. Yokogawa;S. Kusumoto;T. Shiba;I. Yano
研究了 17 种革兰氏阳性菌的细胞壁、肽聚糖和由其中一些制备的水溶性酶消化物以及合成的 N-乙酰胞壁酰-L-丙氨酰-D-异谷氨酰胺 (MDP) 对 ICR 小鼠脾细胞的有丝分裂活性的刺激。除溶壁微球菌外，所有测试的细胞壁，无论其分枝酸含量和免疫增强活性如何，均对脾细胞具有明确的刺激活性。一般来说，分枝杆菌、诺卡氏菌和链霉菌的细胞壁比其他细胞壁具有更强的促有丝分裂活性。细胞壁的有丝分裂活性主要归因于肽聚糖部分，即使肽聚糖被降解为其亚基的单体或二聚体，该活性仍保留，尽管亚基的聚合形式表现出比单体或二聚体更强的活性。还证实了合成 MDP 对 ICR 小鼠脾细胞的明确促有丝分裂作用。讨论了在诺卡氏菌、分枝杆菌和链霉菌的细胞壁中发现的未知原理的起源，这些未知原理负责它们对小鼠脾细胞的有效促有丝分裂活性。
Stimulation of splenocytes from ICR mice by cell walls of 17 species of gram-positive bacteria, and peptidoglycans and water-soluble enzymatic digests prepared from some of them, and by synthetic N-acetylmuramyl-L-alanyl-D-isoglutamine (MDP) was studied in terms of mitogenic activity. All the cell walls tested except those of Micrococcus lysodeikticus, regardless of their mycolic acid content and immunopotentiating activity, had definite stimulatory activity on splenocytes. In general, the cell walls of mycobacteria, nocardia, and stretomyces had stronger mitogenic activity than other cell walls. The mitogenic activity of cell walls was mainly attributable to a peptidoglycan moiety, and the activity was retained even when the peptidoglycan had been degraded into a monomer or a dimer of its subunit, though a polymerized form of the subunits exhibited stronger activity than the monomer or dimer. Definite mitogenicity of synthetic MDP on splenocytes from ICR mice was also confirmed. The origin of the unknown principle(s) that is found in the cell walls of nocardia, mycobacteria and streptomyces and is responsible for their potent mitogenic activity on murine splenocytes was discussed.