Differential expression and cellular distribution of mRNAs encoding alpha- and beta-isoforms of Na(+)-K(+)-ATPase in rat kidney.
Differential expression and cellular distribution of mRNAs encoding alpha- and beta-isoforms of Na(+)-K(+)-ATPase in rat kidney.
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编码Na()-K()-ATPase α-和β-异构体的mRNA在大鼠肾脏中的差异表达和细胞分布。
DOI：
10.1152/ajprenal.1993.265.6.f792
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Kone,BC
中科院分区：
文献类型：
--
作者：
Ahn,KY;Madsen,KM;Tisher,CC;Kone,BC
我们用地高辛标记的cRNA探针进行原位杂交组织化学，系统地研究了正常大鼠肾脏中Na（+）-K（+）-ATP酶α和β亚基亚型mRNA的细胞分布。几乎在所有肾单位节段中都检测到编码α 1-、α 2-、α 3-、β 1-和β 2-亚单位的转录物，在近端小管的S3节段、Henle袢的皮质和髓质粗升支、远曲小管、皮质集合管沿着其全长和肾盂上皮中具有突出的杂交信号。观察到各种亚型的细胞特异性表达模式的几个差异。在α-亚型中，α 3-亚基似乎优先表达于肾小球足细胞和系膜细胞、乳头状间质细胞和肾盂上皮。β-亚型的分布模式也不同，β 1-亚基在肾小球和肾盂上皮中表达程度更高，β 2-亚基优先在乳头状间质细胞和乳头状表面上皮中表达。整个肾脏结构中α和β亚基mRNA的检测和表达模式与六种结构独特的Na（+）-K（+）-ATP酶同工酶的可能性相一致，并表明由α 2-、α 3-和β 2-亚基组成的同工酶在肾钠和钾转运中可能发挥更大的作用。
We have used in situ hybridization histochemistry with isoform-specific, digoxigenin-labeled cRNA probes to characterize systematically the cellular distribution of mRNAs encoding alpha- and beta-subunit isoforms of the Na(+)-K(+)-adenosinetriphosphatase (Na(+)-K(+)-ATPase) in the normal rat kidney. Transcripts encoding the alpha 1-, alpha 2-, alpha 3-, beta 1-, and beta 2-subunits were detected in virtually all of the nephron segments, with prominent hybridization signal in the S3 segment of the proximal tubule, the cortical and medullary thick ascending limb of Henle's loop, the distal convoluted tubule, the cortical collecting duct along its entire length, and the renal pelvic epithelium. Several differences in the cell-specific pattern of expression of the various isoforms were observed. Among the alpha-isoforms, the alpha 3-subunit appeared to be preferentially expressed in the glomerular podocytes and mesangial cells, papillary interstitial cells, and renal pelvic epithelium. The beta-isoforms also differed in their distribution pattern, with the beta 1-subunit expressed to a greater degree in the glomerulus and renal pelvic epithelium and the beta 2-subunit preferentially expressed in the papillary interstitial cells and papillary surface epithelium. The detection and expression pattern of alpha- and beta-subunit mRNAs in structures throughout the kidney is compatible with the possibility of six structurally unique Na(+)-K(+)-ATPase isozymes and suggests a potentially greater role for isozymes comprised of the alpha 2-, alpha 3-, and beta 2-subunits in renal sodium and potassium transport.
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